Role of TLR 9 expression in maternal peripheral blood and placenta in intrauterine transmission of HBV
10.3760/cma.j.issn.0254-6450.2019.09.009
- VernacularTitle: 母源外周血和胎盘组织中Toll样受体9表达在HBV宫内传播中的作用
- Author:
Yuzhang SHAO
1
;
Min YAN
2
;
Ni HU
2
;
Hairong WANG
2
;
Ting FU
2
;
Jie GAO
2
;
Lei ZHANG
2
Author Information
1. Medical College, Xi’an Jiaotong University, Xi’an 710061, China
2. Department of Epidemiology, Key Laboratory of Hazard Assessment and Control in Special Operational Environment of Ministry of Education, School of Military Preventive Medicine, Air Force Military Medical University, Xi’an 710032, China
- Publication Type:Journal Article
- Keywords:
HBV;
TLR 9;
Intrauterine transmission;
Dominate infection;
Occult infection
- From:
Chinese Journal of Epidemiology
2019;40(9):1065-1070
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of TLR 9 in intrauterine transmission of hepatitis B virus (HBV) through blood pathway and placenta.
Methods:Epidemiological investigation was carried out in 290 HBsAg positive parturients and 45 normal parturients (control group) in Northwest Women and Children Hospital of Shaanxi Province. Enzyme-linked immunosorbent assay (ELISA) was used to detect five serological makers of hepatitis B and TLR 9 levels in peripheral blood of pregnant women and newborns. HBV DNA was detected by real-time fluorescence quantitative PCR. Detection of TLR 9 expression in placenta by immunohistochemical method. A case-control study was conducted to analyze the difference of TLR 9 levels in placenta and peripheral blood of HBsAg- positive pregnant women with intrauterine transmission of HBV.
Results:The incidence of dominant HBV infection (DBI), occult HBV infection (OBI) and intrauterine transmission of HBV were 9.28% (27/291), 40.21% (117/291) and 49.48% (144/291) respectively. (1) The level of TLR 9 in peripheral blood of HBsAg-positive parturients, non-HBV intrauterine transmission (NBIT) group and OBI group were significantly lower than that of control group (P<0.001). The level of TLR 9 in DBI group was significantly higher than those in NBIT group and OBI group (P=0.000). (2) The TLR 9 level in HBeAg-negative group was significantly lower than that in HBeAg-positive parturients in OBI group (P=0.01). (3) With the increased severity of intrauterine transmission of HBV in each HBV DNA load group, the TLR 9 level in maternal peripheral blood increased significantly (P<0.05). (4) With the increased severity of intrauterine transmission of HBV, the levels of TLR 9 increased significantly in antiviral therapy, immunoglobulin injection and non-hepatitis B vaccine groups (P<0.05). (5) The expression of TLR 9 in placenta tissues with DBI group was significantly higher than that in OBI group and NBIT group (P<0.05).
Conclusions:HBV can inhibit the secretion of TLR 9 in parturient to some extent, but HBeAg can stimulate the secretion of TLR 9. However, with the increased severity of intrauterine transmission of HBV, the level of TLR 9 in parturients is increased by intra-group cross-differentiation. Therefore, TLR 9 is not an independent marker for screening and grouping, but it can be used as an reference indicator for the monitoring and management of HBsAg-positive parturients.
