Preparation of dual-modal nanoprobe for fluorescent-magnetic imaging with manganese-doped carbon quantum dots and imaging study in tumor bearing mice
10.3760/cma.j.issn.2095-2848.2019.09.006
- VernacularTitle: 锰掺杂－碳量子点荧光－磁共振双模态纳米探针的制备及荷瘤裸鼠成像
- Author:
Huiting XU
1
;
Jianming NI
1
;
Cuiping HAN
2
;
Jingjing LI
2
;
Wenjuan WU
1
;
Chunjuan JIANG
1
;
Kai XU
2
Author Information
1. Department of Radiology, Wuxi Second Hospital Affiliated to Nanjing Medical University, Wuxi 214002, China
2. Department of Radiology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, China
- Publication Type:Journal Article
- Keywords:
Nanoparticles;
Manganese;
Quantum dots;
Optical imaging;
Magnetic resonance imaging;
Ovarian neoplasms;
Tumor cells, cultured;
Mice, nude
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2019;39(9):537-541
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare manganese-doped carbon quantum dots (Mn-CDs) dual-modal nanoprobe for fluorescent-magnetic imaging, and evaluate its characteristics and potential on fluorescence imaging and MRI.
Methods:Mn-CDs were synthesized at 150 ℃. The form, diameter, component, fluorescent capability, T1 relaxation rate, stability and cytotoxicity of Mn-CDs in vivo were verified. The fluorescence imaging of HO-8910 tumor-bearing mice was performed on small animal imager, and the whole-body enhanced imaging was performed on 3.0 T MRI scanner. One-way analysis of variance was used to analyze the data.
Results:The Mn-CDs with the diameter of (4.64±0.85) nm showed a well-defined spherical morphology. The fluorescent spectra of Mn-CDs exhibited that the excitation maximum was at 360 nm and the emission maximum was at 440 nm. The T1 relaxation rate was (3.26±0.04) mmol·L-1·s-1. The Mn-CDs had good stability of fluorescent and magnetic imaging capability at 0, 0.25, 0.5, 0.75, 1.0 and 2 months at room temperature with no significant differences of fluorescent and magnetic signals (F=1.566 and 0.987, both P>0.05). After injection of 200 μl Mn-CDs (15 g/L), mice were all alive and had no viscera damage. The tumor could be observed obviously on fluorescence imaging at 5 min. Enhanced MRI showed that the tumor was unevenly enhanced and Mn-CDs were mainly cleared away through urinary system.
Conclusion:Mn-CDs are stable and have good potential on fluorescence imaging and MRI, which provides a promising multimodal imaging method for tumor detection and monitoring.
