Effect of glutathione on autologous fat graft survival of the rabbits
10.3760/cma.j.issn.1009-4598.2019.09.016
- VernacularTitle: 还原型谷胱甘肽对兔自体脂肪移植存活的影响
- Author:
Liangliang WU
1
;
Li MA
;
Sijing JIANG
;
Daping JIA
;
Fang LIU
;
Tangjun GAO
;
Yu ZHAO
Author Information
1. Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China
- Publication Type:Journal Article
- Keywords:
Glutathione;
Autologous fat grafting;
Tumescent solution;
Oxidative stress
- From:
Chinese Journal of Plastic Surgery
2019;35(9):928-933
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of adding glutathion(GSH) to tumescent solution on autologous fat graft survival.
Methods:14 male and female New Zealand rabbits were divided into experimental group and control group randomly, 7 in each group. Experimental group: The donor areas of the rabbits were injected with 3 ml of tumescent solution with GSH. Control group: The donor areas of the rabbits were injected with 3 ml regular tumescent solution. DCFH-DA probe was used for fluorescent staining of harvested fat cells. Then stained fat cells were measured for the intracellular reactive oxygen species(ROS)content by fluorescence microplate. The grafts were harvested at 3 months after transplantation and assessed by general observation, volume measurement, wet weight measurement, HE staining for the number of fat cells, and CD34 immunohistochemical staining for the measurement of micro-vascular density. T test was performed by using SPSS 24.0.
Results:The intracellular ROS content of harvested fat cells in experimental group was lower than that in control group, and the difference was statistically significant (P<0.05). At 3 months after transplantation, the wet weight of fat grafts in experimental group was (1133.21±87.97) mg and that in control group was (718.79±79.27) mg. The volume of fat grafts in experimental group was (1.00±0.04) ml and that in control group was (0.59±0.03) ml. The number of fat cells in experimental group was (746.6±15.7)/10 high magnification vision and that in control group was (350.1±32.4)/10 high magnification vision. The density in group experimental was (8.1±2.0)/high magnification vision and that in control group was (6.7±2.4)/high magnification vision. The grafts′ volume, wet weight, and the number of fat cells in experimental group were higher than those in control group, and the differences were statistically significant (P<0.05). The difference of micro-vascular density between experimental group and control group had no statistical significance (P>0.05).
Conclusions:The addition of GSH to tumescent solution optimizes the process of autologous fat harvest, thereby improving the survival of fat graft.
