Effect of SALL4 on radiosensitivity of leukemia cell line HL-60
10.3760/cma.j.issn.1004-4221.2019.09.015
- VernacularTitle: SALL4对白血病HL-60细胞放射敏感性影响研究
- Author:
Lin LI
1
;
Guangyin WU
2
;
Xiaoke LI
1
;
Lixiao TIAN
1
;
Yuqing CHEN
3
;
Yan LI
4
Author Information
1. Department of Acute and Critical Medicine, Henan Provincial People′s Hospital, Zhengzhou 450003, China
2. Department of Radiation Oncology, Henan Provincial People′s Hospital, Zhengzhou 450003, China
3. Department of Hematology, Henan Provincial People′s Hospital, Zhengzhou 450003, China
4. Medical Imaging Center, Henan Provincial People′s Hospital, Zhengzhou 450003, China
- Publication Type:Journal Article
- Keywords:
SALL4 gene;
HL-60 cell line;
Radiosensitivity
- From:
Chinese Journal of Radiation Oncology
2019;28(9):706-708
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of down-regulating SALL4 on the radiosensitivity of leukemia cells, aiming to provide new ideas for improving radiosensitivity of leukemia patients.
Methods:Human acute myeloid leukemia cell line HL-60 infected with shRNA SALL4 and shRNA control lentivirus was classified into the Lv-shSALL4 group and Lv-shNC group. The levels of SALL4 mRNA and protein in cells were detected by RT-PCR and Western blot. The infected cells treated with 8 Gy dose irradiation were assigned into the Lv-shSALL4+ radiation and Lv-shNC+ radiation groups. The cell apoptosis was detected by flow cytometry. The levels of cleaved Caspase-3, cleaved Caspase-9 and Bax proteins in cells were determined by Western blot. The cells in the Lv-shSALL4 and Lv-shNC groups were exposed to 0, 2, 4, 6 and 8 Gy irradiation. The radiosensitivity ratio was determined by cell clone test.
Results:The level of SALL4 in the Lv-shSALL4 group was significantly lower than that in the Lv-shNC group (P<0.05). The cell apoptosis rate was significantly increased, the levels of cleaved Caspase-3, cleaved Caspase-9 and Bax proteins were remarkably up-regulated in cells compared with those in the Lv-shNC group (all P<0.05). The cell proliferation ability in the Lv-shSALL4+ radiation was significantly reduced, the cell apoptosis rate was considerably increased, the levels of cleaved Caspase-3, cleaved Caspase-9 and Bax proteins were significantly up-regulated compared with those in the Lv-shSALL4 and Lv-shNC+ radiation groups (all P<0.05). The cell radiosensitization ratio in the Lv-shSALL4 group was 1.323.
Conclusion:Down-regulating SALL4 can increase the radiosensitivity of leukemic cells, inhibit the cell proliferation and induce the apoptosis of leukemic cells.
