The expression and clinical significance of migration inhibitory factor in human pulmonary tissues with chronic obstructive pulmonary disease
10.3760/cma.j.issn.1671-0282.2019.09.012
- VernacularTitle: 巨噬细胞移动抑制因子在慢性阻塞性肺疾病患者肺组织中的表达及意义
- Author:
Bing LI
1
;
Yuanli YANG
1
;
Meixia WANG
1
;
Feng CAO
1
;
Xianfu LIU
1
;
Hongxuan ZHANG
2
Author Information
1. Intensive Care Unit, Longgang Central Hospital of Shenzhen City, Shenzhen 518116, China
2. Eastern Section, Guangdong General Hospital, Guangdong Institute of Geriatrics, Guangzhou 510080, China
- Publication Type:Clinical Trail
- Keywords:
Migration inhibitory factor;
Chronic obstructive pulmonary disease;
Real time-PCR;
ELISA;
Western blot
- From:
Chinese Journal of Emergency Medicine
2019;28(9):1123-1127
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of macrophage migration inhibitory factor (MIF) in pulmonary tissues from patients with chronic obstructive pulmonary disease (COPD) and the relationship with its clinical features.
Methods:One hundred and eighty patients who underwent pulmonary bullectomy lobectomy due to pneumatocele from January 2015 to September 2018 in Longgang Central Hospital were enrolled and classified into patients without COPD (control group)and patients with COPD (COPD group), with 90 patients each group. According to the lung function parameters, 90 patients with COPD were divided into the mild COPD group, the moderate COPD group, and the severe COPD group. The levels of mRNA and protein of MIF were measured with RT-PCR, ELISA and Western blot. One-way ANOVA, Pearson correlation analysis and SNK-q test were used to analyze the results with SPSS 18.0, and P<0.05 was considered statistically significant.
Results:The level of MIF in pulmonary tissues from the control group was obviously lower than those in the COPD group (P<0.05). The level of MIF in pulmonary tissues in the severe COPD group was obviously higher than those in pulmonary tissues in the mild COPD, moderate COPD and control groups (P<0.05). MIF was positively correlated with the lung function parameters (P<0.05).
Conclusion:The high expression of MIF in pulmonary tissues is closely related to the severity of COPD.
