Effect of curcumin on mitochondrial gene and high mobility group box 1 protein in sepsis myocardial injury of rats
10.3760/cma.j.issn.1671-0282.2019.09.008
- VernacularTitle: 姜黄素对大鼠脓毒症心肌损伤线粒体基因及高迁移率族蛋白B1的影响研究
- Author:
Ying LIU
1
;
Ying YU
2
;
Xinping ZHANG
1
;
Shuangjie LI
2
;
Yimin ZHU
3
;
Zhenghui XIAO
1
;
Xiulan LU
1
;
Haipeng YAN
1
Author Information
1. Emergency Center, Hunan Children's Hospital, Changsha 410007, China
2. Hepatology Center, Hunan Children's Hospital, Changsha 410007, China
3. Pediatric Medicine Center, Hunan Provincial People's Hospital, Changsha 410005, China
- Publication Type:Journal Article
- Keywords:
Sepsis;
Myocardial injury;
High mobility group box 1;
Mitochondrial gene;
Curcumin;
Rat
- From:
Chinese Journal of Emergency Medicine
2019;28(9):1100-1105
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of curcumin on myocardial mitochondrial related gene (mtATP6), high mobility group box 1(HMGB1) mRNA expression and plasma HMGB1 level in rats with sepsis, and to explore a new strategy for protection of myocardial injury in sepsis.
Methods:A rat sepsis model was established by cecal ligation and puncture (CLP). Thirty male Sprague-Dawley rats were randomly (random number) divided into the sham operation group (sham group), sepsis group (CLP group), and curcumin intervention group (Cur group), with 10 rats in each group. Rats in the Cur group were treated with curcumin by intraperitoneal injection at 100 mg/kg, and normal saline was injected into rats in the sham and CLP groups. Five rats were randomly (random number) sacrificed at 6 h and 24 h after modeling. The pathological changes of myocardial tissue were observed under light microscope. The levels of CK and CK-MB in serum were detected. The HMGB1 level in plasma was detected by ELISA. Real-time PCR was used to detect the expression of HMGB1 mRNA and mtATP6 in myocardial tissue. One-way ANOVA were performed in multigroup mean comparison, LSD-t test was used to compare the mean values of two samples, and P<0.05 was considered statistically significant.
Results:The pathological score of myocardial tissue in the Cur group was lower than those in the CLP group at 6 h and 24 h (P<0.05). The CK and CK-MB in the Cur group were lower than those in the CLP group at 6 h and 24 h (P<0.05). The levels of HMGB1 in the Cur group were lower than those in the CLP group at 6 h and 24 h (P<0.05). The expression levels of HMGB1 mRNA and mtATP6 in myocardial tissue of the CLP group were lower than those in the sham group at 6 h (P<0.05), while the expression levels of HMGB1 mRNA and mtATP6 in myocardial tissue of the Cur group were higher than those in the CLP group at 6 h, but the difference was not statistically significant (P>0.05).
Conclusion:Curcumin protects acute myocardial injury by affecting HMGB1 release and translocation down-regulation of extracellular levels and regulation of mtATP6 expression in CLP model rats.
