Effects of interferon inducible protein 16 (IFI16) on viral protein and pro-inflammatory cytokine expression in human T-cell leukemia virus type 1 (HTLV-1)-positive T cells

Yuhan Cui; Yuhe Guan; Yue Liu; Ge Zhang; Fan Chen; Mengmeng Chen; Jingliang Sun; Xiaowen Ren; Bo Yang; Jie Wang

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Effects of interferon inducible protein 16 (IFI16) on viral protein and pro-inflammatory cytokine expression in human T-cell leukemia virus type 1 (HTLV-1)-positive T cells

VernacularTitle: IFI16在HTLV-1阳性T细胞中对病毒蛋白和促炎因子表达的影响
Author: Yuhan CUI ¹ ; Yuhe GUAN ^{1
,

2} ; Yue LIU ¹ ; Ge ZHANG ¹ ; Fan CHEN ^{1
,

2} ; Mengmeng CHEN ^{1
,

2} ; Jingliang SUN ^{1
,

2} ; Xiaowen REN ^{1
,

2} ; Bo YANG ^{1
,

3} ; Jie WANG ^{1
,

3}
Author Information

1. Henan Key Laboratory of Immunology and Targeted Drugs, Xinxiang Medical University, Xinxiang 453003, China
2. Scientific Research Innovation Group, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, China
3. Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine in Henan Province, Xinxiang 453003, China
Publication Type:Journal Article
Keywords: Interferon inducible protein 16 (IFI16); Human T-cell leukemia lymphoma virus type 1 (HTLV-1); Interferon; T lymphocyte; TNF-α
From: Chinese Journal of Microbiology and Immunology 2019;39(9):668-673
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effects of interferon inducible protein 16 (IFI16), a cytosolic DNA sensor, on the expression of human T-cell leukemia virus type 1 (HTLV-1) proteins and pro-inflammatory cytokines in adult HTLV-1-positive T cells.
Methods:IFI16 expression in different HTLV-1-positive T cell lines was detected by immunoblot assay. Specific siRNA targeting the IFI16 gene was constructed and the gene silencing efficiency was detected by immunoblot assay. Expression of HTLV-1 Tax protein at mRNA and protein levels was respectively detected by real-time PCR and immunoblot assay after knocking down the expression of IFI16 in HTLV-1-positive T cells with siRNA. Expression of interferon (IFN)-α, IFN-γ, tumor necrosis factor (TNF)-α, Tax and Env were detected by real-time PCR.
Results:Compared with the HTLV-1-negative T cell line Jurkat, IFI16 expression was enhanced in the HTLV-1-positive T cell lines MT2, MT4 and C8166. Tax expression was increased, while that of IFN-α, IFN-γ and TNF-α was decreased in MT2 and MT4 cells after silencing the expression of IFI16 with siRNA.
Conclusions:IFI16 expression was increased in HTLV-1-positive MT2 and MT4 cells. Meanwhile, IFI16 promoted the production of interferon and pro-inflammatory cytokines and inhibited the expression of HTLV-1 proteins.