Genetic characteristics of coxsackievirus A16 isolated in Shenzhen from 2016 to 2017
10.3760/cma.j.issn.0254-5101.2019.09.002
- VernacularTitle: 2016—2017年深圳地区柯萨奇病毒A16型分子特征分析
- Author:
Xiangjie YAO
1
;
Weiqi WANG
2
;
Long CHEN
1
;
Hong YANG
1
;
Jun MENG
1
;
Hong PAN
3
;
Hailong ZHANG
1
;
Hongyu ZHANG
1
;
Renli ZHANG
1
;
Yaqing HE
1
Author Information
1. Institute of Pathogen Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
2. Graduate School, Guangdong Medical University, Dongguan 523808, China
3. Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
- Publication Type:Journal Article
- Keywords:
Coxsackievirus A16;
Hand, foot and mouth disease;
Phylogenetic analysis
- From:
Chinese Journal of Microbiology and Immunology
2019;39(9):652-656
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the genetic characteristics of VP1 genes carried by coxsackievirus A16 strains isolated from cases of hand foot and mouth disease (HFMD) in Shenzhen during 2016 to 2017.
Methods:Fecal and anal swab specimens were collected from patients with mild HFMD in four sentinel hospitals and the Institute of Pathogen Biology, Shenzhen Center for Disease Control and Prevention, China during 2016 to 2017. All specimens were tested for CVA16 viral RNA using real-time RT-PCR. The VP1 genes of 51 randomly selected CVA16 strains were amplified by RT-PCR and then sequenced using TaKaRa Biomedical Technology (Dalian). Bioinformatics software, including Mega6.02, BioEdit and DNAStar, was used for comparison and analysis of the VP1 genes.
Results:CVA16 strains in Shenzhen during 2016 to 2017 mainly belonged to B1a and B1b subtypes as well as an emerging subtype B3. The epidemic of B1b subtype was found in both 2016 (28 strains) and 2017 (19 strains), while the B1a subtype (two strains) was only detected in 2017. Two B3 subtype strains were detected in 2017. The strains of B1b subtype were closely related to the strains isolated in Shanghai (JQ314149), Wenzhou (KP289416) and Beijing (KU254598), while the B1a subtype strains were closely related to the strains isolated in Kunming (JQ316639) and Tailand (GQ184139). The B3 subtype strain was an emerging CVA16 epidemic strain in mainland China. Further comparison of the CVA16 epidemic strains in Shenzhen area during 2016 to 2017 with the CVA16 strains causing severe neurological symptoms showed that two amino acid mutations (S14N and M23L) were found in VP1 protein.
Conclusions:The epidemic strains of CVA16 were B1b subtype in Shenzhen area in 2016. However, B1a, B1b and the emerging B3 subtype strains were prevalent in 2017. Compared with the CVA16 strains causing severe neurological symptoms, the CVA16 strains circulating in Shenzhen during 2016 to 2017 carried two amino acid mutations inVP1 protein.
