Platelet-Rich Fibrin Lysate Can Ameliorate Dysfunction of Chronically UVA-Irradiated Human Dermal Fibroblasts.
10.3349/ymj.2016.57.5.1282
- Author:
Yohanes Widodo WIROHADIDJOJO
1
;
Arief BUDIYANTO
;
Hardyanto SOEBONO
Author Information
1. Department of Dermato-Venereology, Faculty of Medicine, Gadjah Mada University, Sardjito Hospital, Yogyakarta, Indonesia. widiokarsono@yahoo.com
- Publication Type:Brief Communication
- Keywords:
Collagen;
cell migration;
platelet-rich fibrin;
cell proliferation;
ultraviolet-A
- MeSH:
Blood Platelets/*cytology/*metabolism;
Cell Movement/radiation effects;
Cell Proliferation/radiation effects;
Cells, Cultured;
Collagen/metabolism;
Fibrin/*metabolism;
Fibroblasts/*cytology/metabolism/*radiation effects;
Humans;
Skin/*cytology;
Time Factors;
Ultraviolet Rays/*adverse effects
- From:Yonsei Medical Journal
2016;57(5):1282-1285
- CountryRepublic of Korea
- Language:English
-
Abstract:
To determine whether platelet-rich fibrin lysate (PRF-L) could restore the function of chronically ultraviolet-A (UVA)-irradiated human dermal fibroblasts (HDFs), we isolated and sub-cultured HDFs from six different human foreskins. HDFs were divided into two groups: those that received chronic UVA irradiation (total dosages of 10 J cm-2) and those that were not irradiated. We compared the proliferation rates, collagen deposition, and migration rates between the groups and between chronically UVA-irradiated HDFs in control and PRF-L-treated media. Our experiment showed that chronic UVA irradiation significantly decreased (p<0.05) the proliferation rates, migration rates, and collagen deposition of HDFs, compared to controls. Compared to control media, chronically UVA-irradiated HDFs in 50% PRF-L had significantly increased proliferation rates, migration rates, and collagen deposition (p<0.05), and the migration rates and collagen deposition of chronically UVA-irradiated HDFs in 50% PRF-L were equal to those of normal fibroblasts. Based on this experiment, we concluded that PRF-L is a good candidate material for treating UVA-induced photoaging of skin, although the best method for its clinical application remains to be determined.
