Simvasatin plus cyclosporin A inhibits obliterative bronchiolitis in a murine heterotopic tracheal transplant model
10.3760/cma.j.issn.0254-1785.2019.09.011
- VernacularTitle: 辛伐他汀联合环孢素A抑制小鼠异位气管移植模型闭塞性细支气管炎发生
- Author:
Jicheng XI
1
;
Zhibiao ZHANG
;
Chongjun WANG
;
Zheng YAN
;
Di SONG
Author Information
1. Department of Cardiac Surgery, Shanxi Cardiovascular Hospital, Taiyuan 030024, China
- Publication Type:Journal Article
- Keywords:
Lung transplantation;
Statin;
Cyclosporin A;
Bronchiolitis obliterans
- From:
Chinese Journal of Organ Transplantation
2019;40(9):558-562
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of simvastatin combined with cyclosporin A treatment on the development of obliterative bronchiolitis in a murine heterotopic tracheal transplantation model.
Methods:Murine tracheals were heterotopically transplanted from BALB/c donors into C57BL/6 recipients. Transplanted animals received either control chow, chow containing simvastatin, chow containing cyclosporine A, or chow containing simvastatin and cyclosporine A. beginning immediately after transplantation. Epithelial loss and luminal obstruction were analyzed by morphometry. Immunohistochemistry assay was used for quantifying inflammatory cell infiltration and expression of chemokine in tracheal allografts. collagen deposition was studied by picro sirius red staining.Group t test was used to calculate the difference between groups.
Results:simvastatin combined with cyclosporin A treatment reduced chemokine(MCP-1, RANTES)release, inhibited CD4+ and CD8+ T cells and macrophages accumulation in tracheal allografts, resulting in limited bronchial inflammation and diminished epithelial loss. simvastatin plus cyclosporin A treatment also inhibited proliferation of myofibroblast cells, reduced MMP-2 release and decreased the amounts of type I and III collagen deposition, resulting in preserved luminal patency and inhibited development of OB compared with those of controls.
Conclusions:When simvastatin was used in combination with CsA, the development of OB was significantly inhibited.
