Genetic analysis of one family with congenital limb malformations
10.3760/cma.j.issn.1003-9406.2019.09.009
- VernacularTitle: 一个先天性指(趾)畸形家系的基因突变分析
- Author:
Fengying CAI
1
;
Jijun MA
2
;
Rui PAN
3
;
Chao WANG
3
;
Weichao LI
2
;
Chunquan CAI
4
;
Shuxiang LIN
3
;
Jianbo SHU
3
Author Information
1. Department of Physiology, Tianjin Medical College, Tianjin 300222, China
2. Department of Immunology, Tianjin Children’s Hospital, Tianjin 300134, China
3. Tianjin Pediatric Research Institute, Tianjin Children’s Hospital, Tianjin 300134, China
4. Department of Neurosurgery, Tianjin Children's Hospital, Tianjin 300134, China
- Publication Type:Clinical Trail
- Keywords:
Preaxial;
Triphalangeal thumb;
ZPA regulatory sequence;
Mutation
- From:
Chinese Journal of Medical Genetics
2019;36(9):890-892
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect potential mutation in a Chinese pedigree affected with congenital limb malformations.
Methods:Clinical data was collected. Genomic DNA was extracted from peripheral blood samples of family members. The zone of polarizing activity regulatory sequence (ZRS) were amplified by PCR and subjected to direct sequencing.
Results:Among the 13 individuals in this pedigree, there were 4 PPD patients, who were characterized by varying degrees of deformity. The female patients suffered triphalangeal thumb and preaxial polydactyly, while the male patients only had preaxial polydactyly. Only one patient had foot involvement. TA heterogeneous mutations was discovered in the ZRS (105C>G) in all patients, the same mutation was not detected in 2 healthy family members.
Conclusion:The inheritance pattern of PPD was autosomal dominant inheritance. There was a significant variability of symptoms among family patients. The heterozygous mutation of the ZRS (105C>G) probably underlie the disease.
