Evaluation of GeneXpert MTB/RIF and BACTEC-MGIT 960 for the detection of tuberculosis among pneumoconiosis-associated tuberculosis patients
10.3760/cma.j.issn.1001-9391.2019.09.014
- VernacularTitle: GeneXpert MTB/RIF与BACTEC-MGIT 960检测技术在尘肺并发肺结核诊断中的应用
- Author:
Yan JIN
1
,
2
;
Huanqiang WANG
1
;
Jingguang FAN
3
;
Jing PANG
4
;
Peiying ZHANG
4
;
Tao LI
1
Author Information
1. National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100030, China
2. Taizhou Center for Disease Control and Prevention, Taizhou, 318000, China
3. National Center of Occupational Safety and Health, Beijing 102300, China
4. Hunan Chest Hospital, Changsha 410013, China
- Publication Type:Case Reports
- Keywords:
Pneumoconiosis;
Tuberculosis;
GeneXpert MTB/RIF;
Mycobacterium tuberculosis culture (BACTEC-MGIT 960);
Diagnosis
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2019;37(9):690-693
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the performence of GeneXpert MTB/RIF and BACTEC-MGIT 960 on detecting Mycobacterium tuberculosis and rifampicin resistance for pneumoconiosis-associated tuberculosis patients.
Methods:The recruited 133 suspected active pneumoconiosis-associated tuberculosis hospitalized cases, morning sputum samples were collected to do modified L-J culture, conventional proportion method drug susceptibility test, GeneXpert MTB/RIF and BACTEC-MGIT 960. Analyze the sensitivity and specificity of the 133 sputum from patients, the positive rates of patients with tuberculosis in GeneXpert MTB/RIF test, BACTEC-MGIT 960 and modified L-J culture were 37.59%, 34.59% and 30.08% respectively. There was no significant difference among the three tests respectively (P>0.05) . According to the modified L-J culture, the sensitivity of GeneXpert MTB/RIF and BACTEC-MGIT 960 in detecting tuberculosis were 92.5% and 95.0% respectively, and specificity in rifampicin resistance were 86.0% and 91.4% respectively. There was no significant difference between GeneXpert MTB/RIF and BACTEC-MGIT 960 (P>0.05) . According to conventional proportion method drug susceptibility test, the sensitivity of GeneXpert MTB/RIF and BACTEC-MGIT 960 in detecting rifampicin resistance were 90.0% and 100%, and specificity were 92.6% and 96.4%. There was no significant difference between GeneXpert MTB/RIF and BACTEC-MGIT 960 (P>0.05) .
Conclusion:The GeneXpert MTB/RIF has good performence of detecting tuberculosis and rifampicin resistance. It has good application value among pneumoconiosis-associated tuberculosis patients.
