Correlation between gene polymorphism of mannose-binding lectin 2 and posttraumatic susceptibility to sepsis in Hainan Province
10.3760/cma.j.issn.1001-8050.2019.09.011
- VernacularTitle: 甘露糖结合凝集素2基因多态性与海南省创伤患者脓毒症易感性的相关性研究
- Author:
Shaowen HENG
1
,
2
;
Anqiang ZHANG
3
;
Lina XIAN
4
;
Zhihua HU
4
;
Lei PENG
1
,
2
;
Huaping LIANG
3
;
Chuanzhu LYU
5
,
6
Author Information
1. Trauma Center, First Affiliated Hospital of Hainan Medical University, Haikou 570102，China
2. Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Medical University, Haikou 571199，China
3. State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Army Medical University, Chongqing 400042, China
4. Intensive Care Unit, First Affiliated Hospital of Hainan Medical University，Haikou 570102，China
5. Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Medical University, Haikou 571199，China
6. Emergency and Trauma College, Hainan Medical University，Haikou 571199，China
- Publication Type:Journal Article
- Keywords:
Wounds and injuries;
Sepsis;
Mannose-binding lectin 2
- From:
Chinese Journal of Trauma
2019;35(9):829-834
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the clinical relevance of mannose-binding lectin 2 (MBL2) gene polymorphism with traumatic sepsis in Hainan Province.
Methods:A retrospective case control study was conducted to analyze the clinical data of 112 severe trauma patients admitted to the First Affiliated Hospital of Hainan Medical College and Haikou People's Hospital from June 2017 to June 2018. There were 73 males and 39 females, aged 17-83 years [(41.8±8.9)years]. There were 48 patients in the sepsis group and 64 patients in the non-sepsis group. Multiplex single nucleotide extension polymorphism (SNaPshot) typing technique was used to detect the MBL2 gene polymorphism. The correlation between different genotypes and the risk of sepsis was analyzed. ELISA method was used to detect the level of MBL2 in plasma of each group.
Results:Among the three polymorphic loci of MBL2 gene (rs5030737, rs1800450 and rs1800451), the mutation frequency of rs1800450 was 27.7%, while the mutation frequency of rs5030737 and of rs1800451 was 0. The genotype distribution in two groups was in accordance with Hardy-Weinberg equilibrium. The frequency of GA genotype in sepsis group was significantly higher than that in non-sepsis group (P<0.05). A allele frequency in sepsis group was also much higher than that in non-sepsis group (P<0.05). Patients with GA genotype had increased risk of traumatic sepsis when compared to GG genotype(OR=3.442, 95%CI 1.447-8.187). Allele A increased the prevalence of sepsis significantly as well when compared to allele G(OR=2.799, 95%CI 1.270-6.170). The MBL2 level in serum in sepsis patients with genotype GG and GA was significantly lower than that in non-sepsis group (P<0.05). In sepsis group, the MBL2 serum level of patients with genotype GA was obviously lower than that in patients with genotype GG (P<0.05).
Conclusion:MBL2 rs1800450G/A polymorphism is closely related to the occurrence of sepsis in Hainan province, and may be related to the decrease of serum MBL2 level in patients with mutant type.
