Expressions of CD97 isoforms in colon cancer tissues and their clinical significances
10.3760/cma.j.issn.1006-9801.2019.10.004
- VernacularTitle: CD97异构体在结肠癌组织中的表达及其临床意义
- Author:
Pai PENG
1
;
Chaohua HU
1
;
Yuntao HAN
1
;
Yuanbing XU
1
;
Haoyuan SHEN
1
;
Youlin YU
1
;
Hongzhong ZHOU
2
Author Information
1. Department of General Surgery, Xiaogan Hospital Affiliated to Wuhan University of Science and Technology, Xiaogan Central Hospital, Xiaogan 432000, China
2. Department of General Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
- Publication Type:Journal Article
- Keywords:
Colonic neoplasms;
Isoform, CD97;
Reverse transcriptase polymerase chain reaction;
Pathology, clinical
- From:
Cancer Research and Clinic
2019;31(10):662-665
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mRNA expressions of various CD97 isoforms in colorectal carcinoma tissues and their clinical significances.
Methods:A total of 50 colon cancer patients in the First Affiliated Hospital of Wenzhou Medical University from December 2013 to May 2014 and human colon cancer cell lines SW480 and SW620 were enrolled. The real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the mRNA expressions of CD97 human epidermal growth factor (EGF) (1, 2, 5), CD97EGF (1, 2, 3, 5) and CD97EGF (1, 2, 3, 4, 5) in colon cancer tissues, adjacent tissues, normal colon tissues, SW480 cells and SW620 cells. The relationship between the mRNA expression of CD97EGF (1, 2, 5) and the clinicopathological factors was analyzed.
Results:Compared with those low expressions in adjacent tissues and normal tissues, the mRNA expressions of CD97 isoforms CD97EGF (1, 2, 5), CD97EGF (1, 2, 3, 5) and CD97EGF (1, 2, 3, 4, 5) in cancer tissues were highest, and the differences were statistically significant (0.71±0.20 vs. 0.40±0.09 vs. 0.35±0.07, F = 107.642, P < 0.01; 0.45±0.11 vs. 0.26±0.05 vs. 0.27±0.06, F = 94.231, P < 0.01; 0.41±0.10 vs. 0.21±0.05 vs. 0.19±0.03, F = 165.672, P < 0.01). In addition, the mRNA expression of CD97EGF (1, 2, 5) in colon cancer patients was associated with tumor infiltration depth (T1-T2 and T3-T4), clinical stages (Ⅰ-Ⅱ and Ⅲ-Ⅳ), and the differences were statistically significant (t = -2.582, P = 0.013; t = -5.062, P < 0.01). The mRNA expression of CD97EGF (1, 2, 5) in SW620 cells was higher than that in SW480 cells.
Conclusions:CD97 isoforms are highly expressed in colon cancer tissues, and CD97EGF (1, 2, 5) may play an important role in the development and invasion of colon cancer. The CD97 isoforms may be new markers in the treatment of colon cancer.
