Effect of peeling on amygdalin content in Pruni semen
10.3760/cma.j.issn.1673-4246.2019.10.017
- VernacularTitle: 郁李仁去皮对其苦杏仁苷含量的影响
- Author:
Yuan DONG
1
;
Meng ZHONG
;
Congen ZHANG
;
Hao WU
Author Information
1. Department of Chinese Pharmacy, Beijing Friendship Hospital Affiliated to Capital University of Medical Sciences, Beijing 100050, China
- Publication Type:Journal Article
- Keywords:
Pruni semen;
Processing (TCD);
Amygdalin;
Assay (TCD)
- From:
International Journal of Traditional Chinese Medicine
2019;41(10):1111-1114
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The present study aimed to explore the rationality of peeling process of Pruni semen through the determination of amygdalin.
Methods:The content of amarogentin in seed coat, seed kernel and total seed of Pruni semen, respectively, were determined by HPLC according to the methods of content determination under Pruni semen in Chinese Pharmacopeia (2015). The chromatographic column was Inertsil ODS-SP C18 (4.6 mm×150 mm, 5 μm). The gradient elution with acetonitrile-water as mobile phase was performed. The flow velocity was 0.6 ml/min, the column temperature was 40 ℃, and the wavelength was 210 nm.
Results:The average recovery rate (n=6) was 98.26%. The sample was stable within 24 h, and the RSD (n=6) was 1.12%. The quality of control products was good in the range of 0.02-0.40 μg. The analysis indicated that there is no significant differences (P>0.05) in the contents of amygdalin in seed coat, seed kernel and total seed of Pruni semen.
Conclusions:With the content of amygdalin as the index of evaluation, the Pruni semen had better not be peeled.
