Simultaneous determination of emodin, chrysophanol, parietic acid, and magnolol in Fructus Cannabis Bolus by high performance liquid chromatography
10.3760/cma.j.issn.1673-4246.2019.10.016
- VernacularTitle: 高效液相色谱法同时测定麻仁丸中大黄素、大黄酚、大黄酸及厚朴酚含量
- Author:
Xian ZHANG
1
;
Yue LI
Author Information
1. Beijing Institute For Drug Control, Instrument Testing Center, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Assay (TCD);
Fructus Cannabis Bolus;
Emodin;
Chrysophanol;
Rhein;
Magnolol;
High performance liquid chromatography
- From:
International Journal of Traditional Chinese Medicine
2019;41(10):1107-1110
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To simultaneously determinate 4 effective components in Fructus Cannabis Bolus by high performance liquid chromatography (HPLC).
Methods:The column was ALLTIMA-C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol (A)-0.1% phosphoric acid aqueous solution (B) in gradient elution program. The flow rate was 1.0 ml/min, the column temperature was 30℃, and the injection volume was 10 μl. The detection wavelength was set at 254 nm.
Results:The linear ranges of 4 effective components of emodin, chrysophanol, parietic acid, and magnolol were 9.05-81.26 μg (r=0.999 8), 13.28-119.3 μg (r=0.997 6), 8.30-75.40 μg (r=0.998 7), 6.30-61.79 μg (r=0.996 5), respectively; the average recoveries and RSDs of emodin, chrysophanol, parietic acid, and magnolol were 99.25%, 99.46%, 99.51%, 99.86%, respectively. The RSDs of emodin, chrysophanol, parietic acid, and magnolol were 2.04%, 3.23%, 1.84%, and 2.42%, respectively.
Conclusions:The HPLC can be used as an effective and feasible method for the determination of emodin, chrysophanol, parietic acid, and magnolol in Fructus Cannabis Bolus. The method is simple, quick, and reproducible, and can be used for the quality control of Fructus Cannabis Bolus.
