Biological effects of micro RNA-155 in cardiac myocyte apoptosis
10.3760/cma.j.issn.1673-4904.2019.10.014
- VernacularTitle: 微小RNA-155在心肌细胞凋亡中的生物学作用探讨
- Author:
Feng WANG
1
;
Xuanyu ZHANG
1
;
Chunhua LUO
2
Author Information
1. Department of Clinical Laboratory, the People′s Hospital of Wufeng Tujia Autonomous County, Hubei Yichang 443400, China
2. Department of Clinical Laboratory, the First Clinical Medical College of Three Gorges University, the Central People′s Hospital of Yichang, Hubei Yichang 443003, China
- Publication Type:Journal Article
- Keywords:
Myocytes, cardiac;
Apoptosis;
Micro RNA-155
- From:
Chinese Journal of Postgraduates of Medicine
2019;42(10):922-926
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the biological effects of miRNA-155 in the cardiac myocyte apoptosis.
Methods:The mouse-derived macrophage cell line RAW264.7 was treated by different concentration or different stage of oxidized low density lipoprotein (ox-LDL), and transfected by miR-155 mimic (M group), miR-155 mimics-NC (M-NC group), miR-155 inhibitor (I group) or miR-155 inhibitor-NC (I-NC group), respectively. The cell viability was measured by CCK-8 assay, cell apopotosis was measured by TUNEL and flow cytometry.
Results:The ox-LDL induced cell viability of Raw264.7 cells decreased and the expression of miR-155 increased in dose and time dependent manner, after treatment with different concentration of ox-LDL (10, 20, 40, 80, 160 mg/L) or 80 mg/L of ox-LDL with different stage (6, 12, 24, 48, 72 h). The expression of miR-155 increased significantly. Raw264.7 cell viability decreased significantly, compared to that of the blank control. The difference between two groups had statistical significance (P<0.05). The cell viability in M group was significantly higher than that in M-NC group, in I group was significantly higher than that in I-NC group, and the difference between two groups was statistical significance (P<0.05). Compared to that in the M-NC group, the cell apoptosis rate in M group was significantly increased (P<0.05), compared to that in the I-NC group, the cell apoptosis rate in I group were significantly decreased (P<0.05).
Conclusions:miR-155 can enhance the Raw264.7 cell apoptosis induced by ox-LDL.
