Effects of different hemoglobin concentrations on lung injury in a rabbit model of hemorrhagic shock and resuscitation
10.3760/cma.j.issn.0254-1416.2019.10.021
- VernacularTitle: 不同血红蛋白浓度对失血性休克复苏家兔肺损伤的影响
- Author:
Xiaoqian LIU
1
;
Li LIU
1
;
Xun ZHOU
1
;
Nana ZHU
1
;
Yongquan CHEN
2
;
Xiaoju JIN
2
;
Jianrong GUO
1
Author Information
1. Department of Anesthesiology, Gongli Hospital, the Naval Military Medical University, Shanghai 200135, China
2. Department of Anesthesiology, Yijishan Hospital, Wannan Medical College, Wuhu 241000, China
- Publication Type:Journal Article
- Keywords:
Shock, hemorrhagic;
Cardiopulmonary resuscitation;
Hemoglobins;
Acute lung injury
- From:
Chinese Journal of Anesthesiology
2019;39(10):1233-1236
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of different hemoglobin (Hb) concentrations on the lung injury in a rabbit model of hemorrhagic shock and resuscitation (HS-R).
Methods:Fifty healthy male New Zealand rabbits, aged 2 months, weighing 1.9-2.4 kg, were divided into 5 groups (n=10 each) using a random number table method: control group (group C), HS-R group (group H), and three HS-R plus infusion of concentrated red blood cell groups group R1 (60 g/L≤Hb<80 g/L), group R2 (80 g/L≤Hb<100 g/L) and group R3 (100 g/L≤Hb<120 g/L). The animals were sacrificed at 3 h after resuscitation, lung tissues were obtained for examination of the pathological changes with a light microscope, and lung tissues were obtained again for determination of wet to dry weight ratio (W/D ratio), neutrophil myeloperoxidase (MPO), NO level and cell apoptosis (by TUNEL).
Results:Compared with group C, the levels of MAP and NO, W/D ratio and apoptosis index were significantly increased in the other groups (P<0.05). Compared with group H, the levels of MAP and NO, W/D ratio and apoptosis index were significantly decreased in R2 and R3 groups (P<0.05). Compared with group R2, the apoptosis index was significantly increased at T5(P<0.05), and no significant change was found in the other parameters in group R3 (P>0.05).
Conclusion:Maintaining Hb 80-100 g/L after HS-R reduces acute lung injury in rabbits.
