Role of hippocampal nNOS-PSD95 coupling in short-term memory retrieval disorder induced by sevoflurane in mice
10.3760/cma.j.issn.0254-1416.2019.10.013
- VernacularTitle: 海马nNOS-PSD95耦合在七氟醚诱发小鼠短期记忆提取障碍中的作用
- Author:
Xiaoxu YU
1
;
Hong GAO
2
Author Information
1. Anesthesiology School of Guizhou Medical University, Guiyang 550004, China
2. The Third Affiliated Hospital of Guizhou Medical University, Duyun 558000, China
- Publication Type:Journal Article
- Keywords:
Anesthetics, inhalation;
Memory disorders;
Nitric oxide synthase typeⅠ;
Membrane proteins
- From:
Chinese Journal of Anesthesiology
2019;39(10):1199-1202
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the role of hippocampal neuronal nitric oxide synthase (nNOS)-postsynaptic dense protein 95 (PSD95) coupling in short-term memory retrieval disorder induced by sevoflurane in mice.
Methods:Sixteen clean-grade healthy Kunming mice of both sexes, aged 2-3 months, weighing 30-35 g, were divided into 2 groups (n=8 each) according to the random number table method: sevoflurane group (S group) and nNOS-PSD95 uncoupling agent ZL006 group (Z group). After successful establishment of dark avoidance memory, 3.3% sevoflurane and 40% O2 were inhaled for 2 h in both groups, and normal saline 1.5 ml was intraperitoneally injected in group S and ZL006 1 mg/kg in group Z at 30 min before anesthesia.The step-through latency and error times were recorded before anesthesia and at 12 h after the end of anesthesia.The mice were then sacrificed, and hippocampal tissues were taken for determination of the expression of nNOS and PSD95 (by Western blot) and co-expression of nNOS and PSD95 (by immunoprecipitation).
Results:Compared with that before anesthesia, the step-through latency was significantly shortened, and the error times were increased at 12 h after anesthesia in group S (P<0.05), and no significant change was found in the above indicators in group Z (P>0.05). Compared with group S, the step-through latency was significantly prolonged, error times were decreased, the co-expression of nNOS and PSD95 was down-regulated (P<0.05), and no significant change was found in the expression of nNOS and PSD95 in group Z (P>0.05).
Conclusion:The mechanism by which sevoflurane induces short-term memory retrieval disorder may be related to promoting the coupling of nNOS to PSD95 in the hippocampus of mice.
