Effect of NADPH on myocardial ischemia-reperfusion injury in rats
10.3760/cma.j.issn.0254-1416.2019.10.010
- VernacularTitle: NADPH对大鼠心肌缺血再灌注损伤的影响
- Author:
Yifei WANG
1
;
Jiang ZHU
;
Peimin CHEN
;
Liuhui CHANG
;
Hong XIE
Author Information
1. Department of Anesthesiology, the Second Affiliated Hospital of Soochow University, Suzhou 215000, China
- Publication Type:Journal Article
- Keywords:
NADPH;
Myocardial reperfusion injury
- From:
Chinese Journal of Anesthesiology
2019;39(10):1185-1188
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of nicotinamide adenine dinucleotide phosphate (NADPH) on myocardial ischemia-reperfusion (I/R) injury in rats.
Methods:Fifty-six SPF adult male Sprague-Dawley rats, weighing 220-320 g, aged 1-2 months, were divided into 4 groups (n=14 each) using a random number table method: sham operation group (Sham group), myocardial I/R group (I/R group), NADPH group (N group) and diltiazem group (D group). The model of myocardial I/R injury was established by ligation of the left anterior descending branch for 30 min followed by 2-h reperfusion in anesthetized rats.NADPH 16 mg/kg was intravenously infused over 5 min starting from 5 min of reperfusion in N group.Diltiazem 5 mg/kg was infused through the internal jugular vein starting from 10 min before ischemia until the end of ischemia.At 2 h of reperfusion, blood samples were taken from the internal jugular vein for measurement of serum LDH and cTnI concentrations, and myocardial tissues were taken for determination of infarct size and ROS level.
Results:Compared with Sham group, the serum LDH and cTnI concentrations, myocardial infarction size and ROS levels were significantly increased in I/R group (P<0.05). Compared with I/R group, the serum LDH and cTnI concentrations, myocardial infarction size and ROS levels were significantly decreased in N and D groups (P<0.05). Compared with N group, the LDH concentration was significantly decreased (P<0.05), and no significant change was found in the cTnI concentration, myocardial infarction size or ROS level in D group (P>0.05).
Conclusion:NADPH can reduce myocardial I/R injury through antioxidant effect in rats.
