Role of hippocampal CD200R1 in perioperative neurocognitive disorders in mice
10.3760/cma.j.issn.0254-1416.2019.10.009
- VernacularTitle: 海马CD200R1在小鼠围术期神经认知障碍中的作用
- Author:
Danxu MA
1
;
Jinhu LIU
2
;
Wenzhen SHEN
1
;
Changwei WEI
1
;
Chao XIONG
1
;
Dandan LIN
1
;
Ziyi XUE
1
;
Anshi WU
1
Author Information
1. Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
2. Department of Anesthesiology, Beijing First Hospital of Integrated Chinese and Western Medicine, Beijing 100026, China
- Publication Type:Journal Article
- Keywords:
Neurocognitive disorders;
CD200R1
- From:
Chinese Journal of Anesthesiology
2019;39(10):1181-1184
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the role of hippocampal CD200 receptor 1 (CD200R1) in perioperative neurocognitive disorders (PND) in mice.
Methods:Sixty clean-grade male C57BL/6 mice, aged 9-10 months, weighing 32-38 g, were used in the study.The experiment was performed in two parts.Experiment Ⅰ Thirty-six mice were divided into 2 groups (n=18 each) using a random number table method: control group (group C) and PND group.Group C only received isoflurane anesthesia.Partial left lobectomy of the liver was performed under isoflurane anesthesia in group PND.Contextual fear conditioning test was performed at 1, 3 and 7 days after surgery, and the freezing time was recorded.The mice were then sacrificed, and the hippocampus was isolated for determination of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) contents (by enzyme-linked immunosorbent assay) and CD200 and CD200R1 expression (by Western blot). Experiment Ⅱ Twenty-four mice were divided into 2 groups (n=12 each) using a random number table method: CD200-Fc group and IgG1-Fc group.Recombinant proteins CD200-Fc and human IgG1-Fc were injected into the lateral cerebral ventricle in CD200-Fc group and IgG1-Fc group, respectively.Partial left lobectomy of the liver was performed after the end of injection.Contextual fear conditioning test was performed at 1 and 3 days after surgery, and the freezing time was recorded.
Results:Experiment Ⅰ Compared with group C, the freezing time in the contextual fear conditioning test was significantly shortened, and the contents of IL-1β were increased at 1 and 3 days after surgery, the contents of TNF-α were increased at 3 and 7 days after surgery, and the expression of CD200 and CD200R1 was up-regulated at 1 day after surgery in group PND (P<0.05). Experiment Ⅱ Compared with IgG1-Fc group, the freezing time in the contextual fear conditioning test was significantly prolonged at 1 day after surgery in CD200-Fc group (P<0.05).
Conclusion:Up-regulated expression of hippocampal CD200R1 is the endogenous protective mechanism of PND in mice.
