Site-targeted imaging enhancement of viable myocardium after ischemia-reperfusion by a novel nano-scale ultrasound contrast agent: a vivo study
10.3760/cma.j.issn.1004-4477.2019.10.016
- VernacularTitle: 全氟溴辛烷脂质纳米粒对大鼠缺血－再灌注模型中存活心肌的靶向显影研究
- Author:
Yingying LIU
1
;
Mingxing XIE
2
;
Jinfeng XU
1
;
Li ZHANG
2
;
Jing ZHANG
2
;
Feixiang XIANG
2
;
Xiaojuan QIN
2
;
Nan DING
3
;
Chang YANG
3
;
Guangya XIANG
3
Author Information
1. Department of Ultrasonography, Shenzhen People′s Hospital, Second Clinical Medical College of Jinan University, Shenzhen Medical Ultrasond Engineering Center, Shenzhen 518020, China
2. Department of Ultrasonography, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei Provincial Key Laboratory of Molecular Imaging, Wuhan 430022, China
3. College of Pharmacy, Tongji Medical College, Huazhong University of Science and and Technology, Wuhan 430030, China
- Publication Type:Journal Article
- Keywords:
Ultrasound contrast agents;
Nano-scale;
Perfluorocarbon;
Viable myocardium
- From:
Chinese Journal of Ultrasonography
2019;28(10):907-911
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare a kind of lipid nanoparticle ultrasound contrast agents with the ability to target to viable myocardium for diagnosis.
Methods:The agent was a biotinylated, fluorescent-labelled, lipid-coated, liquid perfluorocarbon emulsion. Physico-chemical properties of the agent were measured, including size distribution, Zeta Potential, concentration and so on. Ischemia-reperfusion models were created in rats, and then exposed to biotinylated anti-MCP-1 monoclonal antibody, rhodamine avidin and biotinylated, FITC-labelled nanoparticles, respectively. Echocardiography was taken before and after injection. Frozen sections of their hearts were observed under fluorescence microscope.
Results:The particle diameter, zeta potential and concentration of lipid nanoparticles were (172.30±52.06)nm, (-33.10±6.50)mV and (2.28±0.46)×1011/ml, respectively. From the short-axis view, the myocardium under endocardium of anterior wall was enhanced obviously. While myocardium of other walls were still. The lipid nanoparticles located in the myocardium of anterior wall and gave out bright green and red fluorescence under fluorescence microscope, while neither lipid nanoparticles nor fluorescence were found in other sites of ventricular myocardium.
Conclusions:The viable myocardium can be targeted and acoustically enhanced by the self-made nano-scale ultrasound contrast agent. This new agent has potential to improve sensitivity and specificity for noninvasive identifying viable myocardium.
