Effects of microwave exposure on the enzymatic activity of Trichophyton rubrum
10.3760/cma.j.issn.0254-1424.2019.10.003
- VernacularTitle: 不同辐射功率的微波对红色毛癣菌酶活性的影响
- Author:
Mingjiao TANG
1
;
Fanrong KONG
;
Zhisheng TANG
;
Shaohua YANG
Author Information
1. Department of Rehabilitation Medicine, Affiliated Hospital of Guilin Medical College, Guilin 541000, China
- Publication Type:Journal Article
- Keywords:
Microwaves;
Trichophyton rubrum
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2019;41(10):731-734
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of exposing Trichophyton rubrum fungus to microwaves at different intensities in terms of the activity of succinic dehydrogenase and beta-(1, 3)-D-glucan synthase.
Methods:Trichophyton rubrum organisms were randomly divided into a control group and experimental groups. The experimental groups were incubated at 27 ℃ after direct radiation with 2450 MHz microwaves at 20, 40, 60 or 80 W for 15 min, repeated 8 times. The control group was incubated without any irradiation. Thirty days later, the beta-(1, 3)-D-glucan synthase and succinate dehydrogenase activities were determined using enzyme-linked immunosorbent assays.
Results:The enzymatic activity decreased gradually with increasing radiation intensity. When the output power was 80 W, the beta-(1, 3)-glucan-synthase-D activity was 0.730±0.74 U/ml and that of the succinate dehydrogenase was 1.828±1.774 U/L, both significantly lower than in the groups subjected to less powerful irradiation.
Conclusions:Microwave radiation can decrease the enzymatic activity of Trichophyton rubrum in a dose-dependent manner. Higher intensity is more effective. Microwave irradiation can decrease the activity of succinate dehydrogenase and beta-(1, 3)-glucan synthase from Trichophyton rubrum in vitro, resulting in the destruction of fungal cell walls and interfering with the tricarboxylic acid cycle, furthering cell death. Moreover, the temperature change possibly also helps promote the biological effects of microwave radiation.
