The effects of early treadmill training combined with ultrashort wave irradiation on functional recovery from spinal cord injury
10.3760/cma.j.issn.0254-1424.2019.10.001
- VernacularTitle: 早期跑台训练联合超短波治疗对脊髓损伤大鼠功能恢复的影响
- Author:
Hao SU
1
,
2
;
Shu DU
3
;
Danying CHEN
4
;
Shu CHEN
4
;
Shi SUN
4
;
Yucen WAN
4
;
Zhiqiang WANG
4
;
Lixin ZHANG
4
Author Information
1. China Medical University, Shenyang 110122, China
2. Rehabilitation Center of Shengjing Hospital Affiliated to China Medical University, Shenyang 110134, China
3. Shenyang Emergency Center, Shenyang 110006, China
4. Rehabilitation Center of Shengjing Hospital Affiliated to China Medical University, Shenyang 110134, China
- Publication Type:Journal Article
- Keywords:
Spinal cord injury;
Treadmill training;
Ultrashort wave irradiation;
Auaporin protein-4;
Glial fibrillary acidic protein
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2019;41(10):721-726
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of early-stage training combined with the ultrashort wave therapy on the functional recovery of rats after a spinal cord injury, and to observe the expression of aquaporin protein-4 (AQP-4) and glial fibrillary acidic protein (GFAP).
Methods:Fifty female Sprague-Dawley rats had spinal cord injury (SCI) induced using the modified Allen′s method. After successful modeling, 40 were randomly divided into a sham operation group, a control group, an ultrashort wave group, a treadmill group and a combined group, each of 8. Motor function in their hind limbs was evaluated 4 weeks after the operation using BBB scoring. GFAP and AQP-4 immunohistochemical staining were used to determine the integral optical density (IOD) of the protein expression.
Results:The average BBB score of the sham operation group was 21, while the other four groups averages were all less than 1 on the 1st day after the operation. They gradually increased with time, and by 4 weeks the increases were significant. Compared with the control group at the same time point, the average BBB scores of the treadmill and the combined groups were significantly higher. Compared with the ultrashort wave group, the average BBB score of the treadmill group was higher after 4 weeks, and the combined group′s average was significantly higher at 2, 3 and 4 weeks after the operation. Four weeks after the SCI modeling, the average AQP-4 IOD and GFAP IOD levels of the ultrashort wave group, the treadmill group and the combined group were lower than that of the control group, while the average AQP-4 and GFAP IOD levels of the combined group were significantly lower than those of the ultrashort wave group. Compared with the treadmill group, the combined group had a significantly lower average GFAP level.
Conclusions:Both treadmill training and ultrashort wave treatment promote motor function recovery after a spinal cord injury. The mechanism may be related to downregulation of AQP-4 and GFAP expression at the injured site. Combining the two treatments gives better effects.
