Genotype and evolution of hantavirus in Tiantai of Zhejiang province, 2011-2018
10.3760/cma.j.issn.0254-6450.2019.10.021
- VernacularTitle: 浙江省天台县2011－2018年汉坦病毒基因型别和进化变异研究
- Author:
Pingping YAO
1
;
Gang CHEN
1
;
Fang XU
1
;
Zhangnyu YANG
1
;
Chen CHEN
1
;
Yisheng SUN
1
;
Hangjing LU
1
;
Weilong PANG
2
;
Yun ZHANG
3
;
Hanping ZHU
1
;
Haiqing XIANG
4
Author Information
1. Institute of Microbiological Test, Key Laboratory of Vaccine, Prevention and Control on Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China
2. Tiantai Municipal Center for Disease Control and Prevention, Tiantai 317200, China
3. Institute of Military Medicine, Nanjing Command, Nanjing 210002, China
4. Hangzhou Municipal Center for Health Development, Hangzhou 310001, China
- Publication Type:Journal Article
- Keywords:
Hantavirus;
Genotype;
Gene variation;
Epidemiological characteristic
- From:
Chinese Journal of Epidemiology
2019;40(10):1285-1290
- CountryChina
- Language:Chinese
-
Abstract:
Objective:By investigating the genotype and evolutionary variation of hantavirus (HV) in Tiantai county, a national surveillance site for hemorrhagic fever with renal syndrome (HFRS) was set in Zhejiang province, from 2011 to 2018, to reveal the molecular epidemiological characteristics of hantavirus (HV) in Tiantai.
Methods:Total RNA was extracted from ultrasound treated HV antigen- positive rat lung samples in Tiantai from 2011 to 2018. After cDNA was prepared, nested PCR was used to amplify partial sequence of M fragments by using specific primers of HV. The sequences of HV in Tiantai from 2011 to 2018 were compared with other known HV sequences in order to identify the genotype and analyze the evolution and variation of the virus.
Results:In 67 HV antigen-positive lung specimens, 31 were positive in nested PCR amplification with type-specific primers, including 30 Hantaan virus (HTNV) positive samples, 1 Seoul virus (SEOV) positive sample, and all the 31 samples were from Apodemus agrarius. The phylogenetic tree based on partial M segment was divided into monophyletic group, 30 strains were distributed in HTNV group and 1 was in SEOV group. The HTNV strain Tiantai T2018-130 was independently in one branch, sharing 84.8%-87.9% homology with other strains both at home and abroad, including 29 strains in HTNV group in Tiantai. The other 29 HTNV strains in Tiantai showed closer relationship. The SEOV strain T2016-31 from Apodemus agrarius showed closer relationship with previous strains of SEOV, Tiantai ZT71, ZT10 and Z37 strains of Wenzhou, Zhejiang province.
Conclusions:HTNV, the main genotype of HV in Tiantai of Zhejiang province, showed obvious geographic clustering, but the strain T2018-130 was distinct from the others in Tiantai. Meanwhile, by sequence analysis, we confirmed that The SEOV strain T2016-31 existed in in Apodemus agrarius, indicating there was a phenomenon of "spillover" between virus and host in SEOV evolution.
