Expression and clinical application of SPTBN1 in nasopharyngeal carcinoma
10.3760/cma.j.issn.1009-9158.2019.10.011
- VernacularTitle: βⅡ-血影蛋白在鼻咽癌中的表达及临床应用研究
- Author:
Xulin XIE
1
;
Yunlai LIANG
1
;
Manlin XIANG
1
;
Yupei REN
1
;
Yating MA
1
;
Bin YI
1
Author Information
1. Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China
- Publication Type:Journal Article
- Keywords:
Nasopharyngeal neoplasms;
Spectrin;
Immunoglobulin A
- From:
Chinese Journal of Laboratory Medicine
2019;42(10):869-874
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The expression of spectrin beta chain, brain1(SPTBN1) were measured in nasopharyngeal carcinoma (NPC) cell lines, as well as tissues and serums of NPC cases and normal controls. The clinical value of SPTBN1 expression for NPC diagnosis were assessed along with the antibody levels of early antigen-IgA(EA-IgA) and viral capsid antigen-IgA(VCA-IgA).
Methods:A total of 71 nasopharynx tissue specimens and 130 serum from both NPC cases and matched health controls were collected from December 2016 to December 2018. In logistic regression the levels of SPTBN1, EA-IgA, VCA-IgA were identified and included in an integrative risk prediction model. Discriminatory accuracy was measured by generation of receiver operator curves and estimation of area under the curve (AUC).
Results:The SPTBN1 concentration in cell lines from NP69, 6-10B to 5-8F showed a decreasing trend (H=50.205, P<0.05). The SPTBN1 expression level in serums were significantly lower [73(42, 142)] compared to controls, while EA-IgA and VCA-IgA levels [3.42(1.29, 5.19),3.55(1.95, 5.01)]were higher in NPC serum samples (HSPTBN1=24.105, HEA-IgA=52.398, HVCA-IgA=70.426, P<0.01). The findings were confirmed in tissues that the positive SPTBN1 rate were significantly lower in cases (86.7%) as compared to controls (43.9%, χ2=13.443, P<0.001). In the risk prediction model for NPC diagnosis, the AUC of SPTBN1 alone and the integrated regression model were 0.794 and 0.987, respectively (Z=4.900,P<0.01).
Conclusions:The lower expression of SPTBN1 was a potential marker in NPC diagnosis. The combined analysis of SPTBN1, EA-IgA and VCA-IgA may improve the sensitivity and specificity in NPC diagnosis.
