Establishment and application of an isotope dilution chromatography tandem mass spectrometry reference method for Hcy quantification
10.3760/cma.j.issn.1009-8158.2019.10.009
- VernacularTitle: 同型半胱氨酸同位素稀释液相色谱串联质谱参考测量程序的建立及应用
- Author:
Zhonggan JIN
1
;
Sujie ZHANG
1
;
Yi JU
1
;
Qing LI
1
;
Hewei SUN
1
;
Liping TANG
1
;
Xiaoxuan YU
1
Author Information
1. Shanghai Center for Clinical Laboratory, Shanghai 200126, China
- Publication Type:Journal Article
- Keywords:
Homocysteine;
Chromatography, liquid;
Tandem mass spectrometry;
Reference values;
Reference standards
- From:
Chinese Journal of Laboratory Medicine
2019;42(10):858-863
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a reference measurement procedure for the determination of human serum homocysteine by isotope dilution high performance liquid chromatography-tandem mass spectrometry (ID-LC/MS/MS), and to apply it to establish sample target values for external quality assessment (EQA) in clinical laboratories.
Methods:The reference method of Hcyquantification in our laboratory was establishedaccording to the method recommended by the Joint Committee for Traceability in Laboratory Medicine (JCTLM). The precision, trueness, specificity, residue and matrix effect of the method were evaluated. The reference method was applied to establish Hcy target values for samples of the second EQA in Shanghai of 2018.
Results:The method detects 12.5μmol/L and 37.4μmol/L samples in three batches in three days, and the CV between batches is 1.03% and 2.10%, respectively. The measured values of Standard reference material (SRM) 1955 of National Institute of Standards and Technology (NIST) were within the specified uncertainty range. No matrix effect and carryover were observed. The second EQA data in 2018 showed that the average value of domestic reagent group was lower than that of reference method, and that of imported reagent group was higher than that of reference method.
Conclusion:Thereference measurement procedure of ID-LC/MS/MS was successfully established to determine the human serum homocysteine. It is expected to play a role in tracing the quantities of Hcy in clinical laboratories.
