Correlation between single nucleotide polymorphism of TET2 gene and susceptibility and prognosis of sepsis
10.3760/cma.j.issn.1671-0282.2019.10.016
- VernacularTitle: TET2基因单核苷酸多态性与脓毒症易感性和预后的相关性研究
- Author:
Yan ZHANG
1
;
Xiaoliang WU
1
;
Jinchao HOU
1
;
Baoli CHENG
1
;
Qixing CHEN
2
;
Xiangming FANG
1
Author Information
1. Department of Anesthesiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
2. Clinical Research Center, The Children’s Hospital, Zhejiang University School of Medicine, Hangzhou 310052, China
- Publication Type:Journal Article
- Keywords:
Sepsis;
DNA hydroxymethylase TET2;
Single nucleotide polymorphism (SNP);
Genotype;
Allele;
Case-control study
- From:
Chinese Journal of Emergency Medicine
2019;28(10):1261-1265
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the association of SNPs in TET2 gene with the susceptibility and prognosis of sepsis.
Methods:Ninety-nine patients diagnosed with sepsis and 107 controls were enrolled in the study. The septic patients were further divided into survivors (56 cases) and non-survivors (43 cases) according to the outcome of 28-day hospitalization. Patients without sepsis after major surgery were enrolled as the controls. The genotypes of the five loci (rs6839705, rs7670522, rs7679673, rs7698522 and rs10010325) with high minor allele frequency in the TET2 were screened according to the existing research reports and the SNP database of the NCBI website. The five loci were detected by TaqMan probe based allelic discrimination assays using real-time polymerase chain reaction (PCR). The data were calculated for genetic association study through χ2 test and Fisher’s exact probability method.
Results:There was no significant difference in genotype frequencies of the five tested SNPs in TET2 gene between septic patients and controls or between survivors and non-survivors in septic patients (P > 0.05). Furthermore, the allelic frequencies of the five SNPs between septic patients and controls or between survivors and non-survivors in septic patients also had no significant difference (P > 0.05).
Conclusions:This study showed that the five SNPs in TET2 gene (rs6839705, rs7670522, rs7679673, rs7698522, and rs10010325) were not associated with the susceptibility and prognosis of sepsis, which needs to be further confirmed by large-sample studies.
