Effect of miR-137 on the proliferation and apoptosis of endothelial cells regulated by AKT2 gene
10.3760/cma.j.issn.1003-9406.2019.10.006
- VernacularTitle: miR-137调节AKT2对内皮细胞增殖与凋亡的影响
- Author:
Zemin XU
1
;
Yong LIU
1
;
Na SHEN
2
;
Tao FANG
2
;
Fengshi TIAN
2
Author Information
1. Fourth Center Clinical College of Tianjin Medical University, Tianjin Fourth Central Hospital, Internal Department of Cardiovascular Medicine, Tianjin 300140, China
2. Central Laboratory Metabolic Syndrome Laboratory, Tianjin Fourth Central Hospital, Tianjin 300140, China
- Publication Type:Journal Article
- Keywords:
Vascular endothelial cell;
High glucose;
miR-137;
AKT2 gene;
Apoptosis
- From:
Chinese Journal of Medical Genetics
2019;36(10):975-979
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To assess the effect of miR-137 on the proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) induced by high glucose and its mechanism.
Methods:HUVECs cells were divided into low-glucose group (5.5 mmol/L glucose-treated cells), high-glucose group (33.36 mmol/L glucose-treated cells), anti-NC group (cells treated with 33.36 mmol/L glucose after anti-NC transfection) and anti-miR-137 group (cells treated with 33.36 mmol/L glucose after anti-miR-137 transfection). After 48 hours, qRT-PCR was used to determine the expression of miR-137. CCK-8 assay and flow cytometry were used to detect cell proliferation and apoptosis rate, respectively. The targeting relationship between miR-137 and AKT2 was validated by dual fluorescence reporter gene detection system and AKT2 protein expression after overexpression or inhibition of miR-137.
Results:High glucose could significantly up-regulate the expression of miR-137 in HUVECs cells, and the expression of miR-137 in HUVECs cells transfected with miR-137 inhibitor was significantly decreased (P<0.05). High glucose can significantly inhibit HUVECs cell proliferation and induce apoptosis, while inhibition of miR-137 expression can weaken the effect of high glucose on HUVECs cell proliferation inhibition and apoptosis promotion (P<0.05). Inhibiting AKT2 expression could weaken the inhibitory effect of miR-137 inhibitor on HUVECs cell proliferation and apoptosis (P<0.05).
Conclusion:Inhibiting the expression of miR-137 gene can attenuate the proliferation inhibition and apoptosis promotion of HUVECs induced by high glucose, and the mechanism is related to activating the expression of AKT2.
