The role of DFO in Al (mal) 3-induced ferroptosis in PC12 cells
10.3760/cma.j.issn.1001-9391.2019.10.002
- VernacularTitle: 去铁胺在麦芽酚铝诱导大鼠肾上腺嗜铬细胞瘤细胞铁死亡中的作用
- Author:
Liting CHENG
1
;
Zhuang LI
1
;
Jingjuan REN
1
;
Qiao NIU
2
;
Hongmei YU
3
;
Ruifeng LIANG
1
Author Information
1. Department of Environmental Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, China
2. Department of Occupational Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, China
3. Department of Health Statistics, School of Public Health, Shanxi Medical University, Taiyuan 030001, China
- Publication Type:Journal Article
- Keywords:
Aluminum;
Deferoxamine;
Ferroptosis;
Iron ion;
Oxidative damage
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2019;37(10):722-727
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of Al (mal) 3-induced ferroptosis in rat adrenal pheochromocytoma cells (PC12), to explore the effect of deferoxamine (DFO) .
Methods:Taken PC12 cells growing at logarithmic phase and divided into 6 groups: control group, 200 μmol/L Al (mal) 3 group, 0.5% DMSO group, 200 μmol/L DFO group, Al (mal) 3+DMSO group, Al (mal) 3+DFO group. DMSO and DFO were added to the DMSO group and the Al (mal) 3+DMSO group, the DFO group and the Al (mal) 3+DFO group for 2 h, respectively, Al (mal) 3 was then added to the Al (mal) 3 group, Al (mal) 3+DMSO group, and the Al (mal) 3+DFO group to a final concentration of 200 μmol/L. The cell viability was detected by CCK8, the morphology and ROS levels of PC12 cells was observed by inverted microscope, the cell proliferation toxicity and intracellular iron ion content were detected by colorimetry, the GSH content and GSH-PX activity were detected by biochemical method.
Results:Al (mal) 3 exposure significantly inhibited the growth of PC12 cells and destroyed the cell morphological structure, resulting in increased LDH activity and intracellular iron ion content in PC12 cells, decreased GSH content and GSH-PX activity, increased ROS levels; the combined treatment of Al (mal) 3+DFO can significantly improve the cell viability of PC12 cells, improved cell morphology, decreased cell LDH activity and intracellular iron ion content (P>0.05), increased GSH content and GSH-PX activity, decreased ROS levels.
Conclusion:Al (mal) 3 can induce ferroptosis in PC12 cells, DFO may inhibit ferroptosis by reducing intracellular iron levels and reducing oxidative damage.
