Role of Ad36 in inducing browning of 3T3-L1 cells
10.3760/cma.j.issn.1000-6699.2019.10.011
- VernacularTitle: Ad36诱导3T3-L1细胞棕色化的作用研究
- Author:
Jie LIU
1
;
Xiaodi LIANG
1
;
Yi JIAO
1
;
Xuanyu MENG
1
;
Nuermaimaiti NURBIERYE·
1
;
Tingting HU
1
;
Jiale GAO
1
;
Youzongsheng XU
1
;
Yaqun GUAN
1
Author Information
1. Department of Biochemistry, School of Basic Medical Sciences, Xinjiang Medical University, Urumuqi 830054, China
- Publication Type:Journal Article
- Keywords:
Obesity;
3T3-L1 cells;
Browning;
Cocktail
- From:
Chinese Journal of Endocrinology and Metabolism
2019;35(10):867-873
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of adenovirus type 36 (Ad36) in the browning of 3T3-L1 cells.
Methods:BODIPY staining was performed on the 0, 2nd, 4th, 6th and 8th days of the cocktail induction (control) group and the cocktail plus Ad36 induction (experimental) group to observe the adipogenesis of 3T3-L1 cells.The mRNA and protein expressions of uncoupling protein-1(Ucp1), ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit (Atp5o), cytochrome c oxidase subunit 5B(Cox5b), and perilipin were detected by real-time PCR and Western-blot.
Results:The results of BODIPY staining showed that the lipid droplets in the control group gradually became larger with the differentiation of the cells, while the lipid droplets in the experimental group firstly became larger, and then appeared smaller after Ad36 was added on the fourth day. Compared with the control group, the mRNA and protein expression levels of Ucp1, Atp5o, and Cox5b in the experimental group were significantly increased while the expression level of perilipin was significantly decreased (all P<0.05).
Conclusion:During the differentiation of 3T3-L1 cells, Ad36 promotes its browning via increasing the expressions of Ucp1, Atp5o, and Cox5b.
