Differential expression of long non-coding RNAs in peripheral blood of type 2 diabetic patients
10.3760/cma.j.issn.1000-6699.2019.10.008
- VernacularTitle: 长链非编码RNA在2型糖尿病患者外周血中的表达谱分析研究
- Author:
Xuan ZHU
1
;
Pan ZHANG
1
;
Peian LOU
1
;
Yangguang DU
1
;
Tingjun LIU
1
;
Cheng QIAO
1
;
Zongmei DONG
1
;
Peipei CHEN
1
;
Ting LI
1
Author Information
1. Xuzhou Center for Disease Control and Prevention, Xuzhou 221006, China
- Publication Type:Clinical Trail
- Keywords:
Diabetes mellitus, type 2;
Long non-coding RNAs;
Microarray analysis
- From:
Chinese Journal of Endocrinology and Metabolism
2019;35(10):853-858
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen and verify the differential expression profiles of long non-coding RNAs(LncRNAs) in peripheral blood of patients with type 2 diabetes mellitus(T2DM), to identify the potential molecular specific markers of early T2DM.
Methods:The blood samples of 4 type 2 diabetic patients and 4 normal control subjects were collected for microarray analysis. Then six candidate markers of LncRNAs screened from the differential expression profile were tested by qRT-PCR among the subjects (80 cases in the T2DM group and 50 cases in the control group). The possibility of these LncRNAs as molecular diagnostic markers was analyzed, and finally two of them were carried out by receiver operating characteristic (ROC) analysis.
Results:Compared with control subjects, there were differentially expressed 133 LncRNAs in type 2 diabetic patients, among which 5 were up-regulated with the maximum up-regulated fold 3.29 and 128 were down-regulated with the maximum down-regulated fold 8.99. Six down-regulated LncRNAs were selected for validation and revealed a similar result to that of microarray.The expressions of two LncRNAs(NONHSAT160746 and NONHSAT140069) in peripheral blood of diabetic patients were significantly lower than those of control subjects (P<0.01). The areas under the ROC curve of the two LncRNAs were 0.734 and 0.703, respectively(P<0.01).
Conclusion:LncRNAs NONHSAT160746 and LncRNAs NONHSAT140069 are the potential molecular specific markers for the diagnosis of diabetes mellitus.
