Detection of Helicobacter spp. in gastric, fecal and saliva samples from swine affected by gastric ulceration.
10.4142/jvs.2010.11.3.221
- Author:
Patrizia Casagrande PROIETTI
1
;
Annalisa BIETTA
;
Chiara BRACHELENTE
;
Elvio LEPRI
;
Irit DAVIDSON
;
Maria Pia FRANCIOSINI
Author Information
1. Department of Biopathological Science and Hygiene of Food and Animal Production, Faculty of Veterinary Medicine
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
gastric ulcer;
Helicobacter spp.;
non-H. pylori Helicobacter;
PCR;
swine
- MeSH:
Animals;
Feces/*microbiology;
Helicobacter/*isolation & purification;
Polymerase Chain Reaction/veterinary;
Restriction Mapping/veterinary;
Saliva/*microbiology;
Stomach/*microbiology;
Stomach Ulcer/microbiology/pathology/*veterinary;
Swine;
Swine Diseases/*microbiology/pathology
- From:Journal of Veterinary Science
2010;11(3):221-225
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to evaluate the presence of Helicobacter (H.) spp. in swine affected by gastric ulceration. Stomachs from 400 regularly slaughtered swine were subjected to gross pathological examination to evaluate the presence of gastric ulcers. Sixty-five samples collected from ulcerated pars esophagea and 15 samples from non-ulcerated pyloric portions were submitted to histopathological and molecular analyses, to detect Helicobacter spp., H. suis and H. pylori by PCR. Feces and saliva swabs were also collected from 25 animals in order to detect in vivo the presence of Helicobacter spp.. Gastric ulcers were detected in 373 cases (93%). The presence of ulcers in association with inflammatory processes was further confirmed by histological examination. Forty-nine percent (32/65) of the ulcerated esophageal portions as well as 53% (8/15) of the non-ulcerated pyloric portions were positive for Helicobacter spp. by PCR. The Helicobacter spp. positive samples were also positive for H. suis, while H. pylori was not detected. These results were confirmed by restriction enzyme analysis. With regard to feces and saliva samples, 15/25 (60%) and 16/25 (64%) were positive for Helicobacter spp. PCR, respectively but all were negative in H. suis and H. pylori specific PCR.
