Sequence and phylogenetic analysis of the non-structural 3A and 3B protein-coding regions of foot-and-mouth disease virus subtype A Iran 05.
10.4142/jvs.2010.11.3.243
- Author:
Saber JELOKHANI-NIARAKI
1
;
Majid ESMAELIZAD
;
Morteza DALIRI
;
Rasoul VAEZ-TORSHIZI
;
Morteza KAMALZADEH
;
Mohsen LOTFI
Author Information
1. Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
A Iran 05;
FMDV;
non-structural protein 3A;
non-structural protein 3B;
sequencing
- MeSH:
Amino Acid Sequence;
Base Sequence;
Cluster Analysis;
DNA Primers/genetics;
Foot-and-Mouth Disease Virus/*genetics;
Iran;
Molecular Sequence Data;
*Phylogeny;
Reverse Transcriptase Polymerase Chain Reaction;
Sequence Alignment;
Sequence Analysis, DNA;
Sequence Homology;
Species Specificity;
Viral Nonstructural Proteins/*genetics
- From:Journal of Veterinary Science
2010;11(3):243-247
- CountryRepublic of Korea
- Language:English
-
Abstract:
The A Iran 05 foot-and-mouth disease virus (FMDV) subtype was detected in Iran during 2005 and has proven to be highly virulent. This study was undertaken to focus on molecular and phylogenetic analysis of 3A and 3B coding-regions in the A Iran 05 field isolate. To assess the genetic relatedness of A Iran 05 isolate the nucleotide and predicted amino acid sequences of the 3AB region of type A FMDV isolates were compared with twenty previously described type A FMDV isolates. The phylogenetic tree based on the 672 bp 3AB gene sequences of type A FMDV from thirteen different locations clustered them into five distinct lineages. The A Iran 05 isolate clustered in lineage A along with four type A variants and was closely matched with viruses isolated in Turkey and Pakistan during 2005~2006. The number of protein sequence differences exhibited by each of the isolates revealed that A Iran 05 isolate contains three amino acid substitutions at positions 47 and 119 of 3A and 27 of the 3B coding region. The nucleotide identity between A Iran 05 and the other four isolates of lineage A was estimated to be 98%.
