The Effects of Anti-allergic Ophthalmic Agents on the Cultured Rabbit Conjunctival Cells.
- Author:
Ik Soo BYON
1
;
Jae Sung PARK
;
Ji Eun LEE
;
Jong Soo LEE
Author Information
1. Department of Ophthalmology, College of Medicine, Pusan National University, Busan, Korea. jongsool@pusan.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Anti-allergic agents;
Azelan(R);
Conjunctival cells of rabbit;
LDH;
Patanol(R);
Zaditen(R)
- MeSH:
Anti-Allergic Agents;
Hydrogen-Ion Concentration;
L-Lactate Dehydrogenase;
Microscopy, Electron, Transmission;
Osmolar Concentration
- From:Journal of the Korean Ophthalmological Society
2006;47(8):1340-1348
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the biological effects and cytotoxicity of anti-allergic ophthalmic agents on the cultured conjunctival cells of rabbit in vitro. METHODS: Conjunctival cells of rabbit were exposed to anti-allergic ophthalmic agents. Azelan(R) (Taejoon, Korea), Zaditen(R) (Novartis, USA), Patanol(R) (Alcon, USA) at a concentration 10, 20 and 30% for a period of 30 minutes, 4, 12, and 24 hours respectively. Cell injury assay was performed using lactate dehydrogenase (LDH) leakage assay. We checked the composition, pH, osmolarity of three anti-allergic agents. Light and transmission electron microscopy were performed to compare the cellular damage of rabbit conjunctival cells under various culture treatments. RESULTS: In cultured conjunctival cells of rabbit, the LDH titers increased up to 4 hours after exposure, maintained until 12 hours and then decreased 12 hours after exposed. Azelan(R) and Zaditen(R) showed a higher LDH titer and severe cellular damage of the conjunctival cells, compared with Patanol(R). Of anti-allergic solutions, Azelan(R) and Zaditen(R) revealed markedly lower Na+, Cl- and pH levels than Patanol(R). However, there was no difference in the concentration of preservative or osmolarity of the eye solution among the three anti-allergic agents. CONCLUSIONS: Patanol(R) caused markedly more damage to cultured rabbit conjunctival cells than Azelan(R) and Zaditen(R). If these anti-allergic these agents are clinically used for long periods of time, they may induce the cellular damage of conjunctival cells depending on the composition and pH of anti-allergic drugs.
