Effect of Itopride Hydrochloride on the Ileal and Colonic Motility in Guinea Pig In Vitro.
10.3349/ymj.2008.49.3.472
- Author:
Hyun Chul LIM
1
;
Young Gyun KIM
;
Jung Hyun LIM
;
Hee Sun KIM
;
Hyojin PARK
Author Information
1. Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. HJPARK21@yuhs.ac
- Publication Type:Original Article ; In Vitro ; Research Support, Non-U.S. Gov't
- Keywords:
Itopride;
acetylcholinesterase inhibitor;
dopamine D(2) receptor antagonist
- MeSH:
Animals;
Benzamides/*pharmacology;
Benzyl Compounds/*pharmacology;
Cholinesterase Inhibitors/pharmacology;
Colon/*drug effects/physiology;
Dopamine/pharmacology;
Dose-Response Relationship, Drug;
Gastrointestinal Motility/*drug effects;
Guinea Pigs;
Ileum/*drug effects/physiology;
Neostigmine/pharmacology;
Receptors, Dopamine D1/antagonists & inhibitors/physiology
- From:Yonsei Medical Journal
2008;49(3):472-478
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Itopride hydrochloride (itopride) inhibits acetylcholinesterase (AChE) and antagonizes dopamine D(2) receptor, and has been used as a gastroprokinetic agent. However, its prokinetic effect on the small bowel or colon has not yet been thoroughly investigated. The aim of this study was to investigate the effects of itopride on motor functions of the ileum and colon in guinea pigs. MATERIALS AND METHODS: The distal ileum was excised and the activity of peristaltic contraction was determined by measuring the amplitude and propagation velocity of peristaltic contraction. The distal colon was removed and connected to the chamber containing Krebs-Henseleit solution (K-H solution). Artificial fecal matter was inserted into the oral side of the lumen, and moved toward the anal side by intraluminal perfusion via peristaltic pump. Colonic transit times were measured by the time required for the artificial feces to move a total length of 10cm with 2-cm intervals. RESULTS: In the ileum, itopride accelerated peristaltic velocity at higher dosage (10(-10)-10(-6)M) whereas neostigmine accelerated it only with a lower dosage (10(-10)-10(-9)M). Dopamine (10(-8)M) decelerated the velocity that was recovered by itopride infusion. Itopride and neostigmine significantly shortened colonic transit at a higher dosage (10(-10)-10(-6)M). Dopamine (10(-8)M) delayed colonic transit time that was also recovered after infusion of itopride. CONCLUSION: Itopride has prokinetic effects on both the ileum and colon, which are regulated through inhibitory effects on AChE and antagonistic effects on dopamine D(2) receptor.
