Protective Effect of Ultra Low Molecular Weight Heparin on Glutamate-Induced Apoptosis in Cortical Cells.
10.3349/ymj.2008.49.3.486
- Author:
Tian Gui YU
1
;
Qing Zhu ZHANG
;
Zhi Guo ZHANG
;
Wei Wei WANG
;
Sheng Li JI
;
Guan Hua DU
Author Information
1. Pharmacological Institute of New Drugs, School of Pharmacy, Shandong University, 44 Wen Hua Xi Road, Jinan, P.R. China. sdzhangqz@163.com
- Publication Type:Original Article
- Keywords:
Ultra low molecular weight heparin;
glutamate;
apoptosis;
Ca2+;
caspase-3;
Bcl-2;
Bax
- MeSH:
Animals;
Apoptosis/*drug effects;
Blotting, Western;
Calcium/metabolism;
Caspase 3/metabolism;
Cell Survival/drug effects;
Cells, Cultured;
Cerebral Cortex/cytology;
DNA Fragmentation/drug effects;
Glutamic Acid/*pharmacology;
Heparin, Low-Molecular-Weight/*pharmacology;
Proto-Oncogene Proteins c-bcl-2/metabolism;
Rats;
Rats, Wistar;
bcl-2-Associated X Protein/metabolism
- From:Yonsei Medical Journal
2008;49(3):486-495
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To investigate the effect of ultra low molecular weight heparin (ULMWH) on glutamate induced apoptosis in rat cortical cells and to explore the possible mechanisms. MATERIALS AND METHODS: Cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Apoptosis was first analyzed with Hoechst 33258 and then confirmed by DNA fragmentation. The concentration of free intracellular calcium ([Ca2+](i)) was determined with fura-2/AM fluorometry. The expression of Bcl-2 family protein and caspase-3 were evaluated with Western blot. RESULTS: Typical apoptotic morphological change in rat cortical cells treated with 100micromol/L glutamate for 24h was detected by Hoechst 33258 staining, which was then confirmed by the DNA ladder of agarose gel electrophoresis. The apoptotic rate of the glutamate treated cells was up to 33.21%, and 24 h of treatment with glutamate increased [Ca2+](i), down-regulated Bcl-2 expression, up-regulated Bax expression, and increased caspase-3 activation in rat cortical cells. Our research demonstrated that ULMWH pretreatment can prevent the glutamate- induced apoptosis, attenuate the increase of [Ca2+](i) not only in medium containing Ca2+ but also in Ca2+-free medium, up-regulate the expression of Bcl-2, down-regulate the expression of Bax, and decrease caspase-3 activation. CONCLUSION: ULMWH has neuroprotective capacity to antagonize glutamate-induced apoptosis in cortical cells, through decrease of Ca2+ release and modulation of apoptotic processes.
