Expression of PD-1 in T-cell acute lymphoblastic leukemia cells and its clinical significance
10.3872/j.issn.1007-385x.2019.07.008
- VernacularTitle:PD-1在急性T淋巴细胞白血病细胞中的表达及其临床意义
- Author:
WEN Chunmei
1
;
LI Zixuan
1
;
WANG Yu
1
;
ZHU Xuejun
2
;
MENG Huimin
1
;
JU Jie
1
;
ZHANG Tingting
1
;
ZHANG Xiuyan
1
;
YUAN Lei
3
;
AN Gangli
1
;
YANG Lin
4
,
5
Author Information
1. Cyrus Tang Hematology Center, Soochow University
2. Department of Hematology, Central Laboratory, the Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Provincial Hospital of Traditional Chinese Medicine
3. Department of Hematology, the Third Hospital of Peking University
4. 1a. Cyrus Tang Hematology Center, 1b. Collaborative Innovation Center of Hematology, 1c. State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, Jiangsu, China
5. 2. Persongen Bio Therapeutics (Suzhou) Co., Ltd, Suzhou
- Publication Type:Journal Article
- Keywords:
T-cell acute lymphoblastic leukemia;
programmed death receptor-1;
express;
growth
- From:
Chinese Journal of Cancer Biotherapy
2019;26(7):768-775
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression and clinical significance of PD-1 molecule in tumor cells (T-ALL cells) derived from the patient with T-cell acute lymphoblastic leukemia (T-ALL). Methods: T-ALL cells from one patient and PBMCs from four healthy volunteers provided by the Department of Hematology in Jiangsu Provincial Hospital of Traditional Chinese Medicine in December 2015, and human 293T/PD-1 cells provided by Persongen Bio Therapeutics (Suzhou) Co., Ltd. were used for this study. The mouse T-ALL xenograft model was constructed by injecting T-ALL cells into tail vein of B-NDG mice, and flow cytometry was used to verify whether the cells obtained from the spleen of transplanted mice were mainly consisted of T-ALL cells. Flow cytometry was used to study the protein expression of PD-1 in T-ALL cells, and RT-PCR was applied to further verify the mRNA expression of PD-1 in T-ALL cells. The PD-1 gene in T-ALL cells was sequenced by SNP genotyping to detect whether the DNA sequence of PD-1 gene changed. PD-1 inhibitor was used in vitro to study their effects on proliferation, apoptosis, and the mRNA expression levels of related factors in T-ALL cells. Results: The mouse T-ALL xenograft model was successfully constructed and verified by flow cytometry as T-ALL. PD-1 was highly expressed at both mRNA and protein levels in T-ALL cells (all P<0.01). A C-to-T mutation was detected in the fifth exon of the PD-1 gene. PD-1 inhibitor had no significant effect on proliferation and apoptosis of T-ALL cells in vitro; PD-1 inhibitor up-regulated the mRNA expression of tumor-suppressor protein IGFBP3 and decreased the mRNA expression of oncoprotein SULT1A3 (all P<0.01). Conclusion: PD-1 is highly expressed in T-ALL cells, and PD-1 could be used as a target for clinical diagnosis and treatment for T-ALL.
- Full text:20190708.pdf
