miR-137 inhibits proliferation, invasion and migration of cervical cancer cells by targeting Wnt5a
10.3872/j.issn.1007-385x.2019.07.007
- VernacularTitle:miR-137通过靶向Wnt5a调控宫颈癌细胞的增殖、迁移及侵袭
- Author:
LU Liumei
1
,
2
;
ZHOU Qianjun
1
,
2
;
LI Zilin
1
,
2
;
LI Xuping
1
,
2
Author Information
1. Department of Obstetrics and Gynecology, Dongguan People'
2. s Hospital
- Publication Type:Journal Article
- Keywords:
cervical cancer;
C33Acell;
HeLa cell;
microRNA-137;
Wnt5a;
proliferation;
migration;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2019;26(7):762-767
- CountryChina
- Language:Chinese
-
Abstract:
Objective： ：To investigate the expression of miR-137 in cervical cancer tissues and cells, and to explore its effect on proliferation, migration and invasion of cervical cancer cells as well as the mechanisms. Methods： ：Thirty-two pairs of cervical cancer tissues and corresponding para-cancerous tissues that surgically resected at the Department of Gynecology and Obstetrics of Dongguan People's Hospital from January 2017 to March 2018 were collected for this study. In addition, cervical cancer cell lines C33A, HeLa, SiHa and cervical epithelial immortalized cell line H8 were also collected. The expression of miR-137 in cervical cancer tissues and cell lines was detected by RT-PCR. miR-137 mimics and miR-137 NC were respectively transfected into C33Aand HeLa cells, and the effects of miR-137 over-expression on proliferation, migration and invasion of cervical cancer cell lines were observed by CCK-8 and Transwell assay. Luciferase reporter gene assay and WB were used to determine the relationship between miR-137 and Wnt5a in cervical cancer. Wnt5a over-expression vector was constructed, and the effects of simultaneous over-expression of Wnt5a and miR-137 on proliferation, migration and invasion of C33Aand HeLa cells were observed. Results： ：The expression level of miR-137 was significantly down-regulated in cervical cancer tissues and cell lines, as compared to para-cancerous tissues and H8 cells (all P<0.05). The over-expression of miR-137 significantly inhibited cell proliferation, migration and invasion of C33A and HeLa cells (all P<0.05). Moreover, Wnt5a was identified as a target of miR-137 by luciferase reporter gene assay. Furthermore, Wnt5a over-expression, to a certain degree, attenuated the suppressive effects of miR-137 on the proliferation, migration and invasion of C33A and HeLa cells. Conclusion： ：miR-137 can inhibit the proliferation, migration and invasion of cervical cancer cells via targeting Wnt5a, which may be an effective target for the treatment of cervical cancer.
- Full text:20190707.pdf
