lncRNA LINC00886 over-expression inhibits malignant biological behaviors of esophageal squamous cell carcinoma Eca109 cells
10.3872/j.issn.1007-385x.2019.07.005
- VernacularTitle:过表达lncRNA LINC00886抑制食管鳞状细胞癌Eca109细胞的恶性生 物学行为
- Author:
YANG Liu
1
;
LIANG Jia
1
;
SHEN Supeng
1
;
LIU Lei
2
;
REN Libing
3
;
GUO Wei
1
;
DONG Zhiming
1
Author Information
1. Cancer Institute, the Fourth Hospital of Hebei Medical University
2. Department of Thoracic Surgery, the Fourth Hospital of Hebei Medical University
3. Department of Thoracic Surgery, Central Hospital of Handan City
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma (ESCC);
Eca109 cell;
long non-coding RNA (lncRNA);
LINC00886;
proliferation;
migration;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2019;26(7):751-756
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of lncRNA LINC00886 in human esophageal squamous cell carcinoma (ESCC) tissues and cell lines, and its effects on proliferation, migration and invasion of Eca109 cells. Methods: The cancer tissues and corresponding para-cancerous tissues of 69 ESCC patients were collected in the biological specimen bank of the Fourth Hospital of Hebei Medical University from June 2014 to December 2016; the ESCC cell lines Eca109, TE13, TE1, Kyse150, Yes-2 and Kyse170 were also collected. LINC00886 gene expression in ESCC tissues and cell lines was detected by qPCR. Eca109 cells were transfected with pIRES2-LINC00886 and pIRES2-NC, respectively, and the overexpression efficiency of LINC00886 gene in Eca109 cells was detected by qPCR; MTS, clone formation assay, wound-healing assay and Transwell invasion assay were respectively used to detect the effect of LINC00886 over-expression on proliferation, migration and invasion ability of Eca109 cells. Results: The expression of LINC00886 gene in ESCC tissues was significantly lower than that in para-cancerous tissues (P<0.01), and its expression level was associated with tumor TNM stage and lymph node metastasis (both P<0.05). The expression level of LINC00886 gene in ESCC cell lines was also lower than that of the control group (all P<0.01). Compared with control group, the expression level of LINC00886 gene was significantly higher in Eca109 cells transfected with pIRES2-LINC00886 (both P<0.05). Compared with the control group, LINC00886 overexpression significantly inhibited the proliferation, migration and invasion abilities of Eca109 cells (all P<0.01). Conclusion: The decreased expression of LINC00886 gene may be related to the occurrence and development of ESCC. Over-expression of LINC00886 gene inhibits the proliferation, migration and invasion abilities of ESCC cells.
- Full text:20190705.pdf
