GBX2 over-expression promotes proliferation, migration and invasion of human cervical carcinoma SiHa cells
10.3872/j.issn.1007-385x.2019.08.004
- VernacularTitle:GBX2过表达促进人宫颈癌SiHa细胞增殖、迁移及侵袭
- Author:
WANG Ling
1
,
2
;
LIN Wansong
1
,
2
;
LI Jieyu
1
,
2
;
CHEN Mingshui
1
,
2
;
YE Yunbin
1
,
2
Author Information
1. Immuno-Oncology Laboratory, Fujian Cancer Hospital &
2. Fujian Medical University Cancer Hospital, Fuzhou 350014, Fujian,China； 2. Fujian Key Laboratory of Translational Cancer Medicine
- Publication Type:Journal Article
- Keywords:
GBX2 gene;
cervical cancer;
SiHa cell;
proliferation;
migration;
invasion;
epithelial-mesenchymal transition (EMT)
- From:
Chinese Journal of Cancer Biotherapy
2019;26(8):850-855
- CountryChina
- Language:Chinese
-
Abstract:
Objective: : To investigate the effects of GBX2 gene on the proliferation, migration and invasion of human cervical carcinoma SiHa cells and to explore the mechanism. Methods: Recombinant plasmid over-expressing GBX2 gene (pCMV6-entry-GBX2, experimental group) and empty vector plasmid (pCMV6-entry, negative control group) were transfected into cervical cancer SiHa cells by plasmid transfection technique. The proliferation, colony formation and cell cycle of transfected cells were detected by WST-1 method, Colony formation assay and flow cytometry, respectively. The cell migration and invasion were detected by wound healing assay and Transwell assay. The expression level of IL-6 in cell culture supernatant was detected by ELISA. WB was used to detect the expression changes of EMT-related proteins and to explore its possible mechanism. Results: Compared with the SiHa/pCMV6 negative control group, after up-regulation of GBX2, (1) the proliferation, colony formation, migration and invasion of SiHa/GBX2 cells in the experimental group were significantly enhanced (all P<0.01); The proportion of cells in G0/G1 phase decreased while the proportion of cells in S phase and G2/M phase increased (all P<0.01); (2) the expression of E-cadherin decreased, and the expressions of N-cadherin, vimentin and snail increased (all P<0.01); (3) the expression of IL-6 in the culture supernatant of SiHa/GBX2 cells was significantly up-regulated (P<0.01); (4) STAT3 phosphorylation in SiHa/GBX2 cells was enhanced, and could be inhibited by STAT3 inhibitor S31-201 (P<0.01). Conclusion: GBX2 may induce EMT of cervical cancer SiHa cells through IL-6/STAT3 pathway, thereby promoting the proliferation, migration and invasion of cervical cancer cells.
- Full text:20190804.pdf
