Noscapine inhibits migration of colon cancer SW480 cells by down-regulating Wnt3a/β-catenin signaling pathway and cadherin 17
10.3872/j.issn.1007-385x.2019.10.006
- VernacularTitle:那可丁通过下调钙黏素 17 及 Wnt3a/β-catenin 信号通路抑制结肠癌 SW480细胞迁移
- Author:
HAN Zheng
1
;
HUANG Xiaodong
1
;
LIU Meng
1
;
ZHU Qingxi
1
;
TAN Jie
1
;
LIU Weijie
1
;
CHEN Wei
1
;
ZOU Yanli
1
;
CAI Yishan
1
;
HUANG Shasha
1
;
TIAN Xia
1
Author Information
1. (Department of Gastroenterology, the Third Hospital of Wuhan City
- Publication Type:Journal Article
- Keywords:
noscapine (Nos);
colon cancer;
SW480 cell;
cadherin 17;
Wnt3a/β-catenin signaling pathway;
apoptosis;
migration
- From:
Chinese Journal of Cancer Biotherapy
2019;26(10):1089-1094
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effects of noscapine (Nos) on the expression of cadherin 17 (CDH17) in colon cancer SW480 cells and the mechanism of Nos on cell migration. Methods: SW480 cells were divided into the control group, empty vector (si-EV) group, CDH17 interference (si-CDH17) group, Nos treatment group, and CDH17 interference+Nos treatment (si-CDH17+Nos) group. Small interfering RNA (siRNA) was used to knockdown CDH17, and the selected concentration of Nos was (55.30±2.21) µg/ml (IC50). The mRNA expression of CDH17 was detected by qPCR; the apoptosis and migration abilities of SW480 cells were observed by Hoechst33258 staining and Transwell assay; the contents of VEGF, MMP2 and MMP9 in SW480 cells were measured by ELISA, and the protein expressions of CDH17, Wnt3a and β-catenin were determined by WB. Results: Compared with the control group, mRNA and protein expressions of CDH17 obviously decreased, cell apoptosis and migration significantly reduced, while the contents of VEGF, MMP2 and MMP9 as well as the protein expressions of Wnt3a and β-catenin significantly decreased in Nos treatment group, siCDH17 group and si-CDH17+Nos treatment group (all P<0.01).The effect of si-CDH17+Nos treatment was more significant than that of si-CDH17 (P<0.01). Conclusion: Nos induces apoptosis and inhibits the migration of human colon cancer SW480 cells, which may be related to the down-regulation of CDH17 expression and inhibition of the Wnt3a/β-catenin signaling pathway.
- Full text:20191006.pdf
