Preparation of indocyanine green loaded mesoporous silica nanoparticles and their killing effect on cervical carcinoma HeLa cells
10.3872/j.issn.1007-385x.2019.10.005
- VernacularTitle:载吲哚菁绿二氧化硅纳米颗粒的构建及其对宫颈癌HeLa细胞的杀伤作用
- Author:
NIU Gaoli
1
;
ZHAO Hua
2
,
3
Author Information
1. Department of Gynecology, the 91st Center Hospital of PLA
2. Center for Reproductive Medicine, Henan Provincial People'
3. s Hospital
- Publication Type:Journal Article
- Keywords:
indocyanine green;
mesoporous silica nanoparticles;
drug delivery;
cervical cancer;
HeLa cell
- From:
Chinese Journal of Cancer Biotherapy
2019;26(10):1083-1088
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct indocyanine green-loaded silica nanoparticles (ICG@MSNs) and evaluate their killing effect on cervical cancer HeLa cells. Methods: Mesoporous silica nanoparticles (MSNs) were synthesized by template method, and indocyanine green (ICG) containing photothermal agent was loaded to prepare ICG@MSNs with photothermal effect, which were applied in the research of HeLa cells in vitro. Results: The particle of ICG@MSNs was uniform and in regular spherical shape with the size about 200 nm. ICG@MSNs was similar photothermal effect with pure ICG. Cell endocytosis experiments showed that ICG encapsulated in silica nanoparticles is more likely to be endocytosed by tumor cells, and then played a photothermal role in killing cervical cancer HeLa cells. On the other hand, cytotoxicity experiments showed that under the irradiation of 808 nm laser, ICG@MSNs significantly increased cytotoxicity, which could significantly kill cervical cancer HeLa cells. Conclusion: ICG@MSNs has good stability and biocompatibility, as well as good thermogenesis. It’s photothermal treatment effect on tumor is obvious, which has a good prospect for the treatment of cervical cancer.
- Full text:20191005.pdf
