miR-17-5p regulates proliferation, invasion, migration and apoptosis of nasopharyngeal carcinoma CNE2 cells by down-regulating BRMS1Lexpression
10.3872/j.issn.1007-385x.2019.11.011
- VernacularTitle:miR-17-5p通过下调BRMS1L表达调控鼻咽癌CNE2细胞增殖、侵袭、 迁移和凋亡
- Author:
WANG Jihong
1
;
MA Suli
1
;
XIA Xichao
1
;
ZHANG Xiaoyan
2
;
CHEN Bingqiang
2
Author Information
1. Medical College, Pingdingshan University
2. Department of Otolaryngology, General Hospital of Pingdingshan Shenma Medical Group
- Publication Type:Journal Article
- Keywords:
nasopharyngeal carcinoma;
miR-17-5p;
CNE2 cell;
breast cancer metastasis suppressor 1 like (BRMS1L) gene;
proliferation;
invasion;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
2019;26(11):1249-1255
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the molecular mechanism of miR-17-5p regulating the proliferation and invasion of nasopharyngeal carcinoma cells by regulating the expression of breast cancer metastasis suppressor 1 like (BRMS1-like or BRMS1L) gene. Methods:A total of 40 cases of nasopharyngeal carcinoma tissues and corresponding paracancerous tissues resected from nasopharyngeal carcinoma patients, who were admitted to the General Hospital of Pingdingshan Shenma Medical Group during January 2014 to December 2017, were included in this study; in addition, nasopharyngeal carcinoma cell lines CNE 2, HONE 1, C666-1 and nasopharyngeal immortalized epithelial cell line NP69 were also collected for this study. The expression of miR-17-5p in nasopharyngeal carcinoma tissues and cell lines was detected by qPCR. The targeted relationship between BRMS1L and miR-17-5p was predicted by the StarBase and verified by the Dual luciferase reporter gene assay. Effects of transfection of miR-17-5p mimics and inhibitors on the expression of BRMS1Lin CNE2 cells were detected by WB assay. CCK-8, Transwell and Flow cytometry were used to detect the effects of miR-17-5p/BRMS1L axis on the proliferation, migration, invasion and apoptosis of CNE 2 cells. Results: miR-17-5p was highly expressed in nasopharyngeal carcinoma tissues and cell lines (P<0.05 or P<0.01). Knockdown of miR-17-5p significantly inhibited proliferation, invasion and migration of CNE2 cells but promoted apoptosis (P<0.05 or P<0.01); miR-17-5p targeted BRMS1Land down-regulated its expression. Over-expression of BRMS1Lsignificantly inhibited the proliferation, invasion and migration of CNE2 cells but promoted apoptosis (all P<0.01); while simultaneous over-expression of miR-17-5p and BRMS1L reversed the above effects (all P<0.01). Conclusion: miR-17-5p promoted proliferation, invasion, migration and inhibited apoptosis of CNE 2 cells by down-regulating the expression of BRMS1L.
- Full text:20191111.pdf
