miR-130a-3p inhibits invasion of breast cancer MCF-7 cells through HGF/MET pathway
10.3872/j.issn.1007-385x.2019.11.010
- VernacularTitle:miR-130a-3p通过HGF/MET信号通路抑制乳腺癌细胞MCF-7侵袭
- Author:
LIU Haiwang
1
;
ZHANG Hongxu
2
;
LI Chunhui
1
;
HAO Meiling
1
;
WANG Jun
1
Author Information
1. Department of Pathology, Affiliated Hospital of Chengde Medical College
2. Breast Surgery, Affiliated Hospital of Chengde Medical College
- Publication Type:Journal Article
- Keywords:
breast cancer;
epithelial mesenchymal transition (EMT);
invasion;
metastasis;
miR-130a-3p;
HGF/MET signaling pathway
- From:
Chinese Journal of Cancer Biotherapy
2019;26(11):1243-1248
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the molecular mechanism of miR-130a-3p regulating epithelial mesenchymal transition (EMT) to affect the invasion and metastasis of breast cancer cells through HGF/MET pathway. Methods: A total of 22 pairs of cancer tissues and adjacent normal tissues from breast cancer patients, who were admitted to Affiliated Hospital of Chengde Medical College from January 2018 to October 2018, were collected for this study; in addition, breast cancer cell lines (MCF-7, MDA-MB-231 and MDA-MB453) and normal breast epithelial cells MCF10A were obtained from the Institute of Basic Sciences, Chengde Medical College. And then, the expression of miR-130a-3p in tissues and cell lines were detected by qRT-PCR. The experiment cells were divided into control group, miR-130a-3p mimics group, miR-130a-3p inhibitor group, PHA665752 (a small-molecule MET inhibitor) transfection group and PHA665752+miR-130a-3p inhibitor co-transfection group. CCK-8 assay and Transwell assay were performed to detect the proliferation, invasion and migration of MCF-7 cells, respectively. The expressions of EMT and HGF/MET signaling pathway related proteins in MCF-7 cells were detected by WB. In addition, the targeted relationship between miR-130a-3p and MET was verified by Dual luciferase reporter gene assay. Results: miR-130a-3p was down-regulated in breast cancer tissues and cell lines. Over-expression of miR130a-3p could suppress the proliferation, invasion, migration and EMT of MCF-7 cells, while knockdown of miR-130a-3p had the opposite results. The results of Dual luciferase reporter gene assay indicated that miR-130a-3p targetedly down-regulated the expression of MET, and miR-130a-3p negatively regulated the expression of HGF/MET signaling pathway. Further experiments confirmed that miR-130a-3p inhibited the proliferation, invasion, migration and EMT of MCF-7 cells by blocking HGF/MET signaling pathway. Conclusion: miR-130a-3p suppresses the EMT of MCF-7 cells via blocking HGF/MET signaling pathway, thereby repressing the invasion and metastasis of MCF-7 cells.
- Full text:20191110.pdf
