Polysaccharide of atractylodes macrocephala inhibits the growth of mice in-situ colon cancer HT-29 cell xenograft via activating immune cells
10.3872/j.issn.1007-385x.2019.11.005
- VernacularTitle:白术多糖通过激活免疫细胞抑制小鼠结肠癌HT-29细胞原位移植瘤的 生长
- Author:
FENG Zifang
1
,
2
;
TANG Shihua
1
,
2
;
GUO Lijia
1
,
2
;
HE Ling
1
,
2
;
YANG Ruibin
1
,
2
Author Information
1. Department of Laboratory Medicine, Xingyi People'
2. s Hospital
- Publication Type:Journal Article
- Keywords:
polysaccharide of atractylodes macrocephala (PAM);
colon cancer;
HT-29 cell;
tumor microenvironment;
immune cell
- From:
Chinese Journal of Cancer Biotherapy
2019;26(11):1209-1213
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect and mechanism of polysaccharide of atractylodes macrocephala (PAM) on the growth of colon cancer cells in mice bearing in-situ colon cancer transplantation tumor. Methods: 1×107 colon cancer HT-29 cells labeled with luciferase were injected into colon serosa of the mice to establish the in-situ colon cancer transplantation tumor model. When the tumor volume reached 230 mm3, the mice were given 30 mg/kg PAM (PAM group) or equal volume of normal saline (Control group) by gavage for 10 consecutive days. The effect of PAM on the growth of colon cancer cells in mice was tested by in vivo tumor imaging technology. The expressions of MHCII and IL-12 in granulocytes, dendritic cells and macrophages, the activation of lymphocytes, and IFN-γ expression in CD4+ and CD8+ cells of tumor tissues were detected by Flow cytometry. Results: PAM significantly inhibited the growth of colon cancer cells in mice bearing in-situ colon cancer transplantation tumor (P<0.01). PAM activated immune cells though increasing the expression levels of MHCII and IL-12 in dendritic cells and macrophages (both P<0.01). PAM significantly increased the frequency of CD8+ cells, NK cells, CD44+/NK cells and CD44+/CD4+ cells in tumor tissues and the number of CD8+ cells and NK cells per unit volume (all P<0.01). PAM significantly increased the IFN-γ secretion of CD4+ and CD8+ cells (both P<0.01), too. Conclusion: PAM inhibits the growth of colon cancer by activating immune cells in tumor tissues of mice bearing in-situ colon cancer transplantation tumor.
- Full text:20191105.pdf
