Glycyrrhizin affects malignant biological behaviors of non-small cell lung cancer HCC827 andA549 cells via regulating miR-142/ZEB1 axis
10.3872/j.issn.1007-385x.2019.12.007
- VernacularTitle:甘草酸通过调控miR-142/ZEB1分子轴影响非小细胞肺癌HCC827和 A549细胞的恶性生物学行为
- Author:
ZHAO Runyang
1
,
2
;
MENG Yong
1
,
2
;
WANG Yanmei
1
,
2
;
HOU Congling
1
,
2
Author Information
1. Department of Respiratory, the Second Affiliated Hospital of Henan University of Traditional Chinese Medicine &
2. Henan Hospital of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
glycyrrhizin (GA);
miR-142;
Zinc finger E-box-binding homeobox 1 (ZEB1);
non-small cell lung cancer (NSCLC);
HCC827 cell;
A549 cell;
proliferation;
migration;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2019;26(12):1337-1344
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of glycyrrhizin (GA) on the proliferation, invasion and migration of non-small cell lung cancer HCC827 andA549 cells via regulating miR-142/ZEB1 (Zinc finger E-box-binding homeobox 1) axis. Methods:After being cultured and transfected, HCC827 andA549 cells were divided into 4 groups: NC group (untransfected+3 mmol/L GA), miR-142 inhibitor group (miR-142 knockdown+3 mmol/L GA), pcDNA3.1-ZEB1 group (ZEB1 over-expression+3 mmol/L GA) and pcDNA3.1-ZEB1+ miR-142 mimic group (ZEB1 over-expression+miR-142+3 mmol/L GA). qPCR was used to detect the expression level of miR-142 in HCC827 andA549 cells treated with different concentrations of GA. MTT and Transwell assays were used to examine the proliferation, invasion and migration of HCC827 and A549 cells. WB was used to detect the expression level of ZEB1 protein in HCC827 and A549 cells. Dual-luciferase reporter gene assay was used to explore the relationship between miR-142 and ZEB1. Results: GA significantly inhibited the proliferation, invasion and migration of HCC827 and A549 cells, and up-regulated the expression level of miR-142 ( P < 0.05 or P <0.01). Dual-luciferase reporter gene assay showed that miR-142 could targetedly combine with 3'-UTR of ZEB1 and downregulate the expression of ZEB1 ( P <0.05 or P <0.01). Further experiment validated that GAinhibited ZEB1 expression via up-regulating miR-142, thus suppressed proliferation, invasion and migration of HCC827 and A549 cells ( P <0.05 or P <0.01). Conclusion: GA inhibits the proliferation, invasion and migration of NSCLC HCC827 and A549 cells, the mechanism of which is that GA inhibits the malignant biological behavior of NSCLC HCC827 andA549 cells via up-regulating the inhibition effect of miR-142 on ZEB1.
- Full text:20191207.pdf
