Salidroside regulates DC through TLR4 to increase the lethality of T cells to lung cancer 3LLcells
10.3872/j.issn.1007-385x.2020.01.006
- VernacularTitle:红景天苷通过TLR4调控DC提高T细胞对肺癌3LL细胞的杀伤力
- Author:
ZHANG Xuewei
1
;
ZHANG Yanli
1
;
WEN Zexin
2
;
LI Pengfei
1
;
CUI Lin
1
;
ZHANG Min
1
Author Information
1. Basic Laboratory of the Life Sciences of the Tibet Autonomous, Department of Medicine of Xizang Minzu University
2. Clinical School of Medicine, Medical School of Fujian Medical University
- Publication Type:Journal Article
- Keywords:
salidroside (SAL);
dendritic cells (DCs);
Toll-like receptor 4 (TLR4);
cytotoxic T lymphocyte (CTL)
- From:
Chinese Journal of Cancer Biotherapy
2020;27(1):37-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective： ：To investigatetheeffectofsalidroside(SAL)onthephenotype of dendritic cells (DCs) and the antitumor ability of cytotoxic T lymphocytes (CTL). Methods： ：Lewis lung cancer cell line 3LL, wild type (WT) C57BL/6 mice and TLR4-/- C57BL/6 mice were chosen for this study. Mice bone marrow derived DC precursor cells were obtained to differentiate into immature DCs, which were harvested on the sixth day of culture. CD11c+ DCs were obtained by magnetic beads screening, and further divided into PBS group, SAL group and lipopolysaccharide (LPS) group.After being cultured for 48 h, the effects of SAL on surface molecules and phagocytosis of DCs as well as the efffect of TLR4 pathway on the killing effect of T cells were detected by Fow cytometry. Results： ： Compared with PBS group, expressions of DC surface molecules CD80, CD86 and MHC Ⅱ significantly increased (all P<0.05), phagocytosis significantly decreased (P<0.05), and TLR4 expression level significantly increased (P<0.01) in SAL group; Compared with WT group, after being treated with SAL or LPS, the expressions of DC surface molecules CD80, CD86 and MHC Ⅱ decreased significantly in TLR4-/- group (all P<0.05); ComparedwithPBSgroup,theactivatedCTLinSALgroupexhibited a significantly elevated killing effect against lung cancer 3LLcells (P<0.05). Conclusion：SAL can induce DC maturation by regulating TLR4, thus improving the killing ability of T cells.
- Full text:20200106.pdf
