Tim-3 expression on T cell surface in patients with esophageal cancer and its clinical significance
10.3872/j.issn.1007-385x.2019.03.010
- VernacularTitle:Tim-3在食管癌患者T细胞表面的表达及其临床意义
- Author:
ZHENG Yujia
1
;
YANG Huiyun
2
,
3
;
WU Qian
1
;
WANG Dan
1
;
ZHANG Zhen
2
;
ZHAO Song
4
;
QI Yu
4
;
ZHANG Yi
5
;
HUANG Lan
5
Author Information
1. Biotherapy Center of the First Affiliated Hospital
2. a. Biotherapy Center of the First Affiliated Hospital
3. b. College of Life Sciences
4. Thoracic Surgery of the First Affiliated Hospital, Zhengzhou University,
5. Biotherapy Center of the First Affiliated Hospital, Thoracic Surgery of the First Affiliated Hospital, Zhengzhou University,
- Publication Type:Journal Article
- Keywords:
esophageal cancer;
Tim-3;
T cells;
immune checkpoint
- From:
Chinese Journal of Cancer Biotherapy
2019;26(3):312-316
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of Tim-3 on the surface of T cells in patients with esophageal cancer, and to explore its clinical significance. Methods: Fresh tumor tissues, paracancerous tissues, and peripheral blood were collected from 25 patients with esophageal cancer at the first affiliated Hospital of Zhengzhou University from September 2016 to April 2018. Peripheral blood from 10 healthy subjects was also collected during the same time period. The expressions of Tim-3, early apoptotic molecules and intrinsic factors in tumor tissues and peripheral blood PBMCs of 25 esophageal cancer patients were determined by flow cytometry. Also, the correlation between Tim-3+ T cell proportion and pathological parameters was investigated. The expression of Tim-3 in tumor tissues and paracancerous tissues was detected by fluorescence quantitative real-time polymerase chain reaction (qPCR). TCGA database was used to further verify the expression of Tim-3 in tumor tissues and paracancerous tissues, as well as its relationship with prognosis. Results: Tim-3 expression on T cells was higher in tumor tissues from esophageal cancer patients (P<0.01). Tim-3+ T cell function was in an exhausted status(P<0.05 or P<0.01), and the expression of Tim-3 on the surface of T cells in esophageal cancer microenvironment was closely related to lymph node metastasis and clinical staging (all P<0.01). Conclusion: Taken together, Tim3 expression on the surface of T cells could induce T cell dysfunction in patients with esophageal cancer, suggesting that Tim-3 may serve as a potential therapeutic target for esophageal cancer.
- Full text:20190310.pdf
