miR-19a-3p targets cell adhersion molecule 2 to inhibit proliferation and metastasis of renal carcinoma 7867-O cells via blockingAKT pathway
10.3872/j.issn.1007-385x.2019.03.005
- VernacularTitle:miR-19a-3p靶向细胞黏附分子2阻断AKT通路抑制肾癌细胞786-O的 增殖和迁移
- Author:
YANG Jing
1
;
LU Guoyuan
1
;
SHEN Lei
1
;
SHA Wengang
1
;
SHEN Xiahong
1
;
YANG Aixiang
2
Author Information
1. Department of Nephrology, the First Affiliated Hospital of Soochow University
2. Intensive Care Unit, Suzhou Hospital Affiliated to Nanjing Medical University [North District of Suzhou State Hospital]
- Publication Type:Journal Article
- Keywords:
renal carcinoma;
786-O cell;
miR-19a-3p;
cell adhesion molecule 2 (CADM2);
epithelial mesenchymal transition (EMT)
- From:
Chinese Journal of Cancer Biotherapy
2019;26(3):280-286
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the mechanism of miR-19a-3p regulating cell adhesion molecule 2 (CADM2) to inhibit the proliferation and metastasis of renal carcinoma cells via the AKT signaling pathway. Methods: A total of 42 patients with renal cancer admitted to Department of Nephrology, the First Affiliated Hospital of Suzhou University from April 2012 to November 2017 were enrolled to collect samples of surgically resected renal carcinoma tissues and paracancerous tissues. Expression of miR-19a-3p was detected in renal carcinoma tissues and 4 types of renal carcinoma cell lines such as 786-O by quantitative Real-time polymerase chain reaction (qPCR). The effects of miR-19a-3p knockdown on proliferation, invasion and epithelial mesenchymal transition (EMT) of renal carcinoma 786-O cells were evaluated by CCK-8 assay, Transwell assay and immunofluorescence, respectively. Subsequently, dual luciferase reporter assay was used to verify whether CADM2 was a target gene of miR-19a-3p. Furthermore, Wb was applied to detect the regulatory effect of miR-19a-3p onAKT signaling pathway through CADM2. Results: miR-19a-3p expression was significantly up-regulated in renal carcinoma tissues and cell lines (all P<0.01). Knockdown of miR-19a-3p could inhibit proliferation, invasion and EMT process of 786-O cells; furthermore, the results indicated that CADM2 was a direct target of miR-19a-3p and its expression was down-regulated (P <0.05 or P<0.01). Additionally, knockdown of miR-19a-3p obviously suppressed proliferation, migration and EMT process of 786-O cells via up-regulating CADM2 and blocking AKT pathway (all P<0.05 or P<0.01), thus alleviating the occurrence and development of renal carcinoma. Conclusion: The study demonstrates that miR-19a-3p has a high expression level in renal carcinoma tissues; knockdown of miR-19a-3p could significantly inhibit the proliferation, migration and EMT process of renal carcinoma tissues, and its mechanism may be associated with miR-19a-3p/CADM2/AKT axis.
- Full text:20190305.pdf
